前列腺癌中微卫星不稳定及其临床意义

Relationship between microsatellite instability state with clinical pathological characteristics in prostate cancer

目的:探讨微卫星不稳定(MSI)与前列腺癌临床病理特征关系。方法:选取82例前列腺癌患者的手术切除标本和相应的癌旁组织,采用PCR扩增和变性凝胶电泳-银染法检测MSI,分析其与临床病理特征的关系。结果:82例前列腺癌中,微卫星稳定(MSS)、低微卫星不稳定(MSI-L)及高微卫星不稳定(MSI-H)发生率分别为64.6%(53/82)、25.6%(21/82)、9.8%(8/82)。前列腺癌组织中MSI发生与患者血清PSA和Gleason评分存在相关性(P<0.05),与年龄、TNM分期、有无淋巴结转移等临床病理因素无明显相关性(P>0.05)。结论:MSI前列腺癌侵袭性较低,可能是预后良好的标志。

Objective：To investigate the microsatellite instability（MSI）in the prostate cancer,find the relationship between the MSI and clinicopathological features.Method：The paired samples of tumors and para-carcinoma tissue from 82 prostate cancer patients were analyzed for MSI status using polymerase chain reaction（PCR）,polyacrylamide gel electrophoresis（PAGE）and silver staining.The relationship between MSI status and clinicopathological features and prognosis was assessed.Result：Eight（9.8%）tumors were high-frequency microsatellite instability（MSI-H）,21（25.6%）were low-frequency MSI（MSI-L）and 53（64.6%）were microsatellite stable（MSS）in 82 prostate cancers.The MSI was significantly associated with PSA（P=0.006）and Gleason score（P=0.006）in prostate cancer.MSI has no significant correlation with age and clinical stage.Conclusion：Prostate cancer with MSI is low invasive and potentially has a good prognosis.