海洋石油降解菌Acinetobacter sp.Y9发酵培养基的优化

Optimization of the liquid fermentation medium of the marine petroleum-degrading bacteria Acinetobacter sp. Y9

目的研究发酵培养基主要组分的种类和浓度对海洋石油降解菌Acinetobacter sp.Y9生长的影响。方法采用单因子实验及正交实验对海洋石油降解菌Acinetobacter sp. Y9发酵培养基中的碳源、氮源及磷源进行筛选和优化，并用优化后的发酵培养基在10 L发酵罐中进行Y9菌株的初步发酵培养。结果经优化，Y9菌株的最适发酵培养基配方为：葡萄糖1%，蛋白胨2%，磷酸盐缓冲溶液（磷酸氢二钠＋磷酸二氢钾）0.1 mol/L，氯化钠0.05%，氯化钙0.001%，氯化镁0.019%。在10 L发酵罐中培养12 h后，OD600值达8.24。结论优化后的发酵培养基可用于Y9菌株的中试发酵生产，提高了菌体产量，为大规模发酵生产奠定了基础。

ObjectiveTo investigate the impact of the types and concentrations of major medium components on the growth of the marine oil-degrading bacterial strain Acinetobacter sp. Y9.MethodsCarbon, nitrogen and phosphorus sources in the medium of Y9 fermentation were screened and optimized by using single factor and orthogonal experiments, and the optimized medium was applied for initial fermentation of Acinetobacter sp. Y9 in the 10 L fermentor.ResultsAfter optimization, the optimal fermentation medium of Y9 included 1% glucose, 2% peptone, 0.1 mol/L phosphate buffer solution（Na2HPO4·12H2O ＋ KH2PO4）, 0.05% NaCl2, 0.001% CaCl2 and 0.019 % MgCl2. The value of OD600 of Y9 reached 8.24 after cultivation in the optimized medium in the 10 L fermentor for 12 hours.ConclusionsThe optimized medium could be used in the pilot fermentation production of Y9 strain, which might lay a solid foundation for large scale fermentation production of Y9.