副交感神经抑制或阻断对变应性鼻炎Th2优势的影响

Inhibition of parasympathetic nerve lowered the Th2-predominant inflammation in the nasal mucosa of allergic rhinitis

目的副交感神经抑制是否影响变应性鼻炎(AR)鼻黏膜Th1/Th2细胞因子及神经肽的表达。方法 24只小鼠随机分成对照组、AR模型组和AR治疗组。检测各组白细胞介素4、干扰素γ,P物质、血管活性肠肽的表达。结果 AR模型组白细胞介素4、P物质、血管活性肠肽表达水平明显高于对照组,AR治疗组上述因子低于AR组。结论抑制胆碱能神经可以缓解AR症状,而且可以调节AR鼻黏膜Th2优势的免疫反应以及神经肽等炎症相关因子的表达。

OBJECTIVE To explore the effect of parasympathetic inhibition on the expression of Thl/Th2 cytokines and neuropeptides in nasal mucosa of allergic rhinitis. METHODS Twenty-four mice were randomly divided into three groups： control, AR model, AR model with ipratropium bromide treatment. AR model was established by using ovalbumin sensitization. Ovalbumin serum sIgE was measured by ELISA. The expression of interleukin-4（IL-4）, interferon-gamma（IFN-γ）, substance P（SP）, and vasoactive interstinal peptides（VIP） in nasal mucosa was detected by immunohistochemistry, western blots, and imaging analysis. RESULTS Data from immunohistochemistry and western blot showed that the expression level of IL-4, SP, VIP was significantly higher in AR model than that in control（P〈0.05）, and significantly decreased in AR model with ipratropium bromide treatment（P〈0.05）. CONCLUSION Inhibition of cholinergic nerves can not only relieve the symptoms of allergic rhinitis, but also can modulate the Th2-predominant immune reaction in AR and the expression of neuropeptides.