摘要
目的探讨建立变应性鼻炎(AR)动物模型时,采用不同的观察时长(5分钟和10分钟)对动物模型进行症状评估的差别。方法用卵清蛋白致敏及激发BALB/c小鼠,建立AR模型,症状评分及血清卵清蛋白特异性IgE均证实模型建立成功。采用不同的观察时长对动物的各项症状进行评分,然后对评分结果进行分析。结果实验组动物模型的症状评分与空白对照组比较有显著性差异,建模成功;5分钟时症状评分和10分钟时症状评分在打喷嚏、流清涕、挠鼻等三项症状及总分的评估中无显著性差异。结论可以采用5分钟的评分时长,既可以有效的筛选出AR动物,又可以减少实验时间,增加实验的准确性,减少动物的生理差异带来的误差。
OBJECTIVE To find the differences of symptom assessment in allergic rhinkis（AR） mice at 5 rain and 10 rain intervals. METHODS The AR mice model was established by ovalbumin（OVA）. Symptom score and serum OVA specific IgE confirmed the success of the model. The AR mice＇ symptoms were observed and scores were recorded at 5 min and 10 rain intervals, then the differences were analyzed. RESULTS 5 minutes interval was effective in the symptom assessment of AR models compared with 10 minutes interval, which showed same result as the 5 minutes interval. CONCLUSION 5 minutes interval can be used in the assessment of the AR model＇s symptom. It can sift AR mice models more effectively ,save time and reduce error.
作者
高娴
李娜
张继生
何凡
GAO Xian;LI Na;ZHANG Jisheng;HE Fan(Department of Otolaryngology Head and Neck Surgery, Qingdao Municiple Hospital, Qingdao, Shandong, 266071, China;Department of Otolaryngology Head and Neck Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China;Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, 310051, China)
出处
《中国耳鼻咽喉头颈外科》
CSCD
2018年第4期186-188,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
关键词
鼻炎
变应性
常年性
鼻炎
变应性
季节性
疾病模型
动物
症状和体征
观察
时间
症状评分
Rhinitis, Allergic, Perennial
Rhinitis, Allergic, Seasonal
Disease Models, Animal
Signs and Symptoms
Observation
Time
symptom score
