基于SSR标记的海带、裙带菜的遗传构成分析

Analysis of the Genetic Composition in Saccharina japonica and Undaria pinnatifida Based on SSR Markers

旨在通过对配子体克隆和孢子体幼苗的初代筛选,以培育出纯度更高、性状更优良的海带与裙带菜品种。以11个海带与裙带菜克隆组合为材料,在筛选出区分亲本♀与♂的数对引物的基础上,对其子代与亲本的遗传构成进行了分析。试验中共选出13对能够区分11个组合亲本♀与亲本♂的SSR标记,分析结果显示,2013年育苗的48号组合中所有子代都是其亲本的后代,49号组合中只有1个子代是其亲本的后代;2014年育苗的22号组合中所有子代都是其亲本的后代;2015年育苗的海带与裙带菜杂交组合中,36号、38号、39号与41号杂交成功,其他组合未杂交成功;2016年育苗的59号组合中有7个子代与亲本无亲缘关系。分析这11个组合中所有个体与亲本的遗传构成发现:部分个体是其亲本的后代,部分个体遗传构成与亲本不一致。

Saccharina japonica and Undaria pinnatifida varieties with higher purity and better character that could be cultivated bythe first generation screening of gametophyte and spore seedlings. Using 11 seedling combinations of S. japonica and u. pinnatifida and basedon a number of primers that may distinguish male and female parents of each group,the genetic composition of their parents and progenywere analyzed. Thirteen pairs of SSR markers which were able to distinguish male and female parents of 11 combinations were selected inthe experiment. Results showed that among the 2013 seedlings,all progenies were the offspring of their parents in No. 48 combination,butonly one of progenies was the offspring of their parents in No. 49 combination. Parents were genetically identical to all progenies in No. 22 combination of 2014 seedlings. Among all hybridized combinations of S. japonica and u. pinnatifida in 2015 seedlings No.36,No.38,No.39,No.41 were hybridized successfully,while other combinations were not so. There were no biological relationships between seven of progeniesand their parents in No. 59 combination of 2016 seedlings. Analyzing the genetic composition of all individuals and parents in these 11 combinations revealed that some individuals were progenies of their parents,but the genetic composition of some individuals were inconsistentwith their parents.