葡萄籽中白藜芦醇的提取及其对LO2细胞氧化损伤保护及延缓衰老作用

Resveratrol Extraction from Grape Seed and Its Effect on LO2 Cell Oxidative Damage Protection and Anti-aging

通过超声波辅助双水相法提取葡萄籽中白藜芦醇,酒精(100 mmol/L)诱导LO2细胞构建氧化损伤模型,D-半乳糖构建衰老小鼠模型,采用白藜芦醇提取物干预后,对细胞及小鼠肝匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)、丙二醛(MDA)含量进行测定。在液料比为1∶10、浸提时间40 min、超声温度50℃、超声功率300 W、超声时间10 min,双水相体系为(乙醇∶硫酸铵=1∶1)白藜芦醇提取率达到2.408±0.088 mg/g。白藜芦醇提取物作用LO2细胞后,能够降低乙醇诱导引起的丙二醛含量升高,能显著提高SOD和GSH-px的活性。白藜芦醇提取物对衰老模型小鼠肝中丙二醛的产生有一定的抑制作用,且能够提升SOD和GSH-px的活性。

Resveratrol was extracted from grape seed by ultrasonic assisted two-phase aqueous method. The oxidative damage model wasconstructed by inducing LO2 cell with alcohol（100 mmol/L）,and aging mice model was built via D-galactose. The superoxide dismutase（SOD）,glutathione peroxidase（GSH-px）activities and malondialdehyde（MDA）content in the cells and mice liver homogenate were measuredafter intervention by resveratrol extracts. Resveratrol extraction yield was 2.408±0.088 mg/g when liquid ratio was 1∶10,leaching time was40 min,ultrasonic time,power and temperature were 300 W,10 min and 50℃,and aqueous two-phase system was ethanol ：ammoniumsulphate = 1∶1. Resveratrol extracts affecting LO2 cell reduced cells MDA content and increased the activity of SOD and GSH-px induced byethanol. Moreover,resveratrol extracts presented a certain inhibitory effects on liver MDA production,and increased the activities of SOD andGSH-px in aging model mice.