HIF-2α和Securin作为乳腺癌术后无瘤生存率预测指标的临床研究

Expression of HIF-2α and Securin for Predicting Disease-free Survival of Patients with Breast Cancer

[目的]探讨缺氧诱导因子-2α（HIF-2α）与securin在乳腺癌组织中的表达及其对无瘤生存率的意义。[方法]采用免疫组化SP法检测70例乳腺癌组织中HIF-2α和securin的表达,并分析其与乳腺癌临床疾病特征的关系及相关性。对患者进行无瘤生存期的随访,Kaplan Meier生存曲线分析HIF-2α和securin对无瘤生存期的意义。[结果 ]70例乳腺癌组织中HIF-2α和securin阳性率、高表达率分别为30.0%（21/70）和34.3%（24/70）。Spearman相关分析表明,HIF-2α与securin的表达呈正相关（r=0.381,P=0.001）。Securin高表达和HIF-2α阳性与乳腺癌患者绝经情况、雌激素受体、孕酮受体、Her-2、淋巴结转移、ki67、TNM分期和肿瘤大小均无关（P〉0.05）。Kaplan Meier生存曲线表明,HIF-2α阳性患者的无瘤生存期明显高于阴性患者。Cox模型多因素生存分析结果显示,HIF-2α是乳腺癌无瘤生存期的独立影响因素（P〈0.05）。[结论]HIF-2α和securin与乳腺癌的发生、发展密切相关,尤其是HIF-2α可能成为判断乳腺癌术后无瘤生存率的独立有效指标。

[Objective]To investigate the expression of HIF-2α and securin in breast cancer and its relation to disease-free survival.[Methods]The expression of HIF-2α and securin was detected by SP immunohistochemistry in 70 patients with breast cancer.The disease-free survival（DFS）of patients was examined by Kaplan-Meier survival curve and the relationship of HIF-2α and securin expression with clinical features and DFS was analyzed.[Results]The expressions of HIF-2α and securin were 30.0%（21/70）and 34.3%（24/70）in breast cancer,respectively.Spearman correlation analysis revealed that HIF-2α and securin were significantly correlated with each other（r=0.381,P=0.001）.The expression of HIF-2α or securin was not correlated with menopausal status,PR,ER,HER-2,lymphatic metastasis,Ki67,TNM and size of tumor（all P 〉0.05）.KaplanMeier survival curve showed the DFS of patients with HIF-2α positive expression was higher than those with negative expression.Cox regression model analysis revealed that HIF-2α was an independent prognostic factor of disease free survival（P 〈0.05）.[Conclusion]The expression of HIF-2α was closely correlated with securin in breast cancer.HIF-2α may be an independent protective factor of disease-free survival in postoperative patients with breast cancer.