IL-4和IL-13在甲状腺乳头状癌中的表达及其与临床病理特征和预后的关系

Expression of IL-4 and IL-13 in thyroid papillary carcinoma and their relationship with clinicopathological parameters and prognosis

目的:探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)肿瘤组织中IL-4和IL-13的表达情况及其与临床病理特征、患者术后复发(包括转移)的关系。方法:收集经病理证实的PTC及癌旁组织标本185例,另取正常甲状腺组织20例作为对照组。采用免疫组织化学法检测组织中IL-4和IL-13蛋白表达情况。结果:癌组织中IL-4蛋白的阳性表达率为95.68%,显著高于癌旁组织37.84%和正常组织5.00%(P<0.05)。癌组织中IL-13蛋白的阳性表达率为90.81%,显著高于癌旁组织41.08%和正常组织0.00%(P<0.05)。IL-4和IL-13表达情况与肿瘤最大直径、浸润深度、分化程度、TNM分期等临床特征均有明显相关性(P<0.05)。IL-4高表达组术后复发率显著高于IL-4低表达组,差异有统计学意义(χ~2=5.491,P=0.019)。IL-13高表达组术后复发率显著高于IL-13低表达组,差异有统计学意义(χ~2=4.345,P=0.037)。COX回归分析结果显示:IL-4高表达和IL-13高表达是影响PCT患者术后复发的独立危险因素(P<0.05)。结论:IL-4和IL-13在PTC肿瘤组织中表达上调,与肿瘤最大直径、浸润深度、分化程度和TNM分期有关,IL-4和IL-13在肿瘤组织中的高表达是术后复发(包括转移)的独立危险因素。

Objective： To investigate the expression of IL-4 and IL-13 in papillary thyroid carcinoma（PTC） and its relationship with clinicopathological features and postoperative recurrence（including metastasis）. Methods： A total of 185 cases of pathologically confirmed papillary thyroid carcinoma and adjacent tissues were collected. Another 20 cases of normal thyroid tissue as a control group. Immunohistochemistry was used to detect the expression of IL-4 and IL-13 in the tissues. Results： The positive rate of IL-4 protein in cancer tissues was 95.68%, which was significantly higher than that in adjacent tissues 37.84% and normal tissues 5.00%（P〈0.05）. The positive rate of IL-13 protein in cancer tissues was 90.81%, which was significantly higher than that in adjacent tissues 41.08% and normal tissues 0.00%（P〈0.05）. The expression of IL-4 and IL-13 had significant relationship with clinical features such as tumor size, depth of invasion, differentiation and TNM staging（P0.05）. The recurrence rate of high IL-4 expression group was significantly higher than that of low IL-4 expression group（χ^2=5.491, P=0.019）. The recurrence rate of IL-13 high expression group was significantly higher than that of IL-13 low expression group, the difference was statistically significant（χ^2=4.345, P=0.037）. COX regression analysis showed that high expression of IL-4 and high expression of IL-13 were independent risk factors for postoperative recurrence in patients with PCT（P0.05）. Conclusion： IL-4 and IL-13 in PTC tumor tissue are upregulated, and are related to tumor size, depth of invasion, differentiation and TNM staging, and the high expression of IL-4 and IL-13 in tumor tissue is an independent risk factor for postoperative recurrence（including metastasis）.