淋巴滞留性脑病大鼠血管周围间隙扩张的研究

Study on the Dilatation of Perivascular Space in Rats with Lymphatic Stagnation Encephalopathy

目的:观察大鼠淋巴滞留性脑病对血管周围间隙(VRS)的影响,探索VRS扩张的机制与临床意义。方法:取健康SD雄性大鼠40只,体重(250±10)g,将其分为24h对照组、48h对照组、24h和48h模型组,各组均为10只。用阻断颈部淋巴引流的方法制备大鼠淋巴滞留性脑病模型。分别于24h、48h用4%多聚甲醛灌注固定并处死取脑,病理观察并统计分析。结果:模型组大鼠脑组织H-E染色后,表现为VRS明显扩张,呈椭圆形或圆形,VRS环绕在血管周围,VRS透明腔隙中含有组织淋巴液。模型组扩张的VRS数目为(10.65±4.10)个,对照组扩张的VRS数为(1.95±1.10)个,差异明显。模型组与对照组VRS截面积分别为(6.17±2.49)μm^2和(0.39±0.28)μm^2,差异有统计学意义。结论:对双侧颈淋巴结进行结扎和摘除,会导致全脑淋巴回流受阻,从而导致全脑VRS扩张和脑组织肿胀。在全脑淋巴回流受阻后,可出现扩张VRS数目和截面积增加的情况,说明VRS扩张的重要机制之一就是全脑淋巴回流受阻,VRS有望作为临床疾病的潜在指标。

Objective： To observe the effect of lymphatic encephalopathy（VRS） on the peripheral blood vessel（VRS） in rats and explore the mechanism and clinical significance of VRS expansion. Methods： 40 healthy SD male rats were selected, and the weight（250±10） g was divided into 24 h control group, 48 h control group, 24 h and 48 h model group, with 10 groups. A model of lymphatic occluded brain disease in rats was prepared by blocking the lymphatic drainage of the neck. The cerebral, pathological observation and statistical analysis were also observed in the fixation and execution of 4% polyformaldehyde perfusion in 24 h and 48 h respectively.Results： After the h-e staining of the rat brain in the model group, the expression of the VRS was significantly dilated, which was elliptical or circular, and VRS was surrounded by the vessels, and the VRS transparent cavity had tissue lymphatic fluid. The number of VRS expanded in the model group was（10.65±4.10）, and the number of VRS in the control group was（1.95±1.10）. The difference was obvious. The area of the model group and the control group was（6.17±2.49） μm^2 and（0.39±0.28） μm^2, and the difference was statistically significant. Conclusion： The ligation and removal of bilateral cervical lymph node can lead to obstruction of whole brain lymphatic reflux, leading to VRS expansion and swelling of brain tissue. After whole brain lymph is blocked, can appear expansion VRS number and cross-sectional area increases, that one of the important mechanisms of VRS expansion is whole brain lymph circumfluence suffocate suffocate, VRS is expected as a potential indicator of clinical disease.