胃祺饮水提液抑制人脐静脉血管内皮细胞生长和小管形成作用研究

Inhibition effects of water extract of Weiqi Decoction on growth and tube formation in human umbilical vein endothelial cells

目的探讨胃祺饮水提液对人脐静脉内皮细胞(HUVECs)生长和管腔形成能力的影响及其分子机制。方法取人脐静脉内皮细胞(HUVECs)分为6组,分别采用0、0.125、0.250、0.500、1 mg·ml^(-1)的胃祺饮水提物和30μmol/L的5-FU进行培养。培养24h,采用CCK-8检测不同浓度的胃祺饮方对HUVECs细胞增殖的影响;Matrigel实验检测HUVECs管腔形成能力;Western blot检测MAPK通路相关蛋白(ERK、p38)及其磷酸化水平的蛋白表达。结果胃祺饮水提液能够抑制HUVECs细胞的存活率,并能够剂量依赖性抑制HUVECs细胞管腔形成能力(P<0.01),且胃祺饮水提液能够显著下调HUVECs细胞MAPK通路相关蛋白(ERK、p38)及其磷酸化水平(P<0.01)。结论胃祺饮水提液可抑制HUVECs细胞的存活率和管腔形成能力,其机制可能与下调MAPK通路相关蛋白(ERK、p38)的磷酸化水平有关。

Objective To investigate the effects and molecular mechanisms of water extract of Weiqi Decoction on the growth and tube formation of human umbilical vein endothelial cells（ HUVECs）. Methods The HUVECs were divided into 6 groups and treated with water extract of Weiqi Decoction at concentrations of 0,0.125,0.25,0.5 and 1 mg·ml^-1 or 5-FU at concentration of 30 μmol/L,respectively. After 24 hours culture,the effects of Weiqi Decoction at different concentrations on the cell proliferation of HUVECs were detected by CCK-8 assay. The tube formation of HUVECs was detected by Matrigel assay. The protein expressions and phosphorylation levels of MAPK pathway-related proteins（ ERK and p38） were detected by Western blot. Results The water extract of Weiqi Decoction could inhibit the survival rate of HUVECs. In addition,the water extract of Weiqi Decoction could inhibit the tube formation of HUVECs in a dose-dependent manner（ P〈0.01）,and significantly down-regulate the protein expressions and phosphorylation levels of MAPK pathway-related proteins（ ERK and p38） in HUVECs（ P〈0.01）. Conclusion The water extract of Weiqi Decoction can inhibit the survival rate and tube formation of HUVECs. The mechanism may be associated with the down-regulation on the expressions and phosphorylation levels of MAPK pathway related proteins（ ERK and p38）.