顺铂诱导肺癌A549细胞保护性自噬及肺岩宁对自噬蛋白Atg5、Atg7的影响

Cisplatin induced protective autophagy in A549 lung cancer cells and effects of Feiyanning decoction on expressions of autophagy proteins Atg5 and Atg7

目的观察顺铂作用肺癌A549细胞后自噬的发生,及肺岩宁对自噬相关蛋白Atg5、Atg7的影响。方法顺铂和肺岩宁处理肺癌A549细胞,CCK-8筛选顺铂和肺岩宁的优化给药浓度,CCK-8检测各组A549细胞增殖能力,Western blot检测各组自噬蛋白表达。结果与单药顺铂相比,3 mg/L的顺铂联合20 mmol/L的自噬抑制剂氯喹明显提高了A549细胞增殖的抑制率(P<0.05)。同时,顺铂导致了微管相关蛋白1轻链3Ⅱ型(microtubule-associated protein 1 light chain 3,LC3Ⅱ)蛋白表达增加(P<0.01)。肺岩宁干预A549细胞后,自噬蛋白Atg5蛋白表达低于模型组和顺铂组(P<0.05),Atg7蛋白表达显著低于模型组和顺铂组(P<0.01)。结论顺铂诱导了A549细胞产生保护性自噬,肺岩宁可能通过抑制肺癌细胞自噬来发挥抗肺癌细胞增殖作用。

Objective To observe the autophagy induced by cisplatin in A549 lung cancer cells and the effects of Feiyanning decoction on the autophagy related proteins including autophagy related gene 5（ Atg5） and autophagy related gene 7（ Atg7）. Methods The A549 lung cancer cells were cultured and treated with cisplatin and Feiyanning decoction. The optimized concentrations of cisplatin and Feiyanning were screened by cell counting kit-8（ CCK-8） assay. The proliferation ability of A549 cells in each group was measured by CCK-8 assay. The expressions of autophagy related proteins in each group were detected by Western blot.Results Compared with cisplatin alone,3 mg/L cisplatin combined with 20 mmol/L chloroquine（ autophagy inhibitor） significantly increased the inhibition rate of A549 cell proliferation（ P〈0.05）. Meanwhile,the protein expression of microtubule-associated protein 1 light chain 3（ LC3 Ⅱ） was increased after cisplatin treatment（ P〈0.01）. The Atg5 protein expression in the Feiyanning group was lower than that in the model group and the cisplatin group（ P〈0.05）,and the Atg7 protein expression in the Feiyanning group was significantly lower than that in the model group and the cisplatin group（ P〈0.01）. Conclusion Cisplatin induces protective autophagy in A549 lung cancer cells,and Feiyangning may suppress the cell proliferation by inhibiting autophagy in lung cancer cells.