常用胆汁类动物药中特征离子的探索研究及应用

Study of characteristic ions in frequently-used traditional Chinese medicinal materials derived from animal bile and its application

采用液质联用的方法对常用的胆汁类动物药的特征离子进行研究，以期解决该类药材及含有该类药材的复方制剂专属性检验的问题。通过UPLC．Q-TOF对样品进行测定，然后使用MarkerLynxTMv．4．1质谱数据处理软件以及胆汁酸类成分的选择离子色谱图对数据进行对比分析，结果方法对于猪胆粉、熊胆粉、体外培育牛黄和人工牛黄的专属性鉴别效果较好，在护肝片和人工牛黄甲硝唑胶囊的专属性鉴别中效果也较好。该方法适用于猪胆粉、熊胆粉、体外培育牛黄和人工牛黄以及一些含上述药材的复方制剂的专属性鉴别。

A LC-MS method was established for study of characteristic ions in frequently-used traditional Chinese medicinal materi- als derived from animal bile. UPLC-Q-TOF was used in the data acquisition work, then, the software of MarkerLynxTM v. 4. 1 was per- formed in the chemometric analysis of data. Besides, selected ion chromatograms of these bile acid ions were comparative studied. Bet- ter results were gained in the specificity identification of pig bile, bear bile, cultivated cow-bezoar in vitro, artificial cow-bezoar and some compound preparations （ Hugan tablet and Rengongniuhuang Jiaxiaozuo capsule） by this method. The method is suitable tor the specificity identification of pig bile, bear bile, cultivated cow-bezoar in vitro, artificial cow-bezoar and compound preparations contai- ning these medicinal materials.