软骨下钻孔联合白及胶复合物修复关节软骨缺损的研究

Subchondral drilling method combined with gum-bletilla complex to repair articular cartilage defects

用成年新西兰大白兔构建A，B两类关节软骨缺损动物模型。A模型为未钻通软骨下骨模型，采用同体对照，右侧为（种子细胞＋白及胶＋PluronicF-127）复合支架材料修复；左侧为空白对照。B模型为钻通软骨下骨模型，同体对照，右侧为（元细胞白及胶＋PluronicF-127）复合支架材料修复；左侧为空白对照。另设一组采用B模型动物，用PluronicF-127人工合成材料修复，双侧相同。在术后4，12，24周处死动物，取修复区组织作HE染色、Ⅱ型胶原免疫组化染色、阿新蓝染色和甲苯胺蓝染色，光镜下观察，参照Wakitanis组织学评分标准，对各组动物修复组织形态学进行半定量评分。A，B2组动物软骨缺损模型，均为透明软骨修复，结果满意。无细胞白及胶与PluronicF．127可注射型复合支架材料，与有种子细胞＋自及胶＋Plu-tonicF-127复合物支架材料，二者对关节软骨缺的损修复效果无明显差异，有白及胶的人工合成材料优于单纯人工合成材料的修复，提示白及胶在复合支架材料修复关节软骨缺损中起了莺要作用。

Two types （A model and B model） of articular cartilage defect models were prepared by using adult New Zealand white rabbits. A model group was applied by drilling without through subchondral bone, whose right joint was repaired by composite scaffolds made by seed cell, gum-bletilla as well as Pluronic F-127, and left side was blank control： B model group was applied by subchondral drilling method, whose right joint was repaired by using composite scaffolds made by gum-bletilla and Pluronic F-127 without seed cells, and left side was blank control. Autogen0us contrast was used in both model types. In addition, another group was applied with B model type rabbits, which was repaired with artificial complex material of Pluronic F-127 in both joint sides. 4, 12 and 24 weeks af- ter operation, the animals were sacrificed and the samples were collected from repaired area for staining with HE, type Ⅱ collagen im- munohistochemical method, AIcian blue, and toluidine blue, and then were observed with optical microscope. Semi-quantitative scores were graded by referring to Wakitanis histological scoring standard to investigate the histomorphoiogy of repaired tissue. Hyaline carti- lage repairing was achieved in both Group A and Group B, with satisfactory results. There were no significant differences on repairing effects for articular cartilage defects between composite scaffolds made by seed cell, gum-bletilla and Pluronic F-127, and the compos- ite scaffolds made by gum-bletilla and Pluronic F-127 without seed cell. Better repairing effects for articular cartilage defects were ob- served in groups with use of gum-bletilla, indicating that gum-bletilla is a vital part in composite scaffolds material.