临床样本中水貂嵌杯病毒的全基因组分析

Genome Analysis of the Mink Calicivirus Detected in Clinical Sample

为分析水貂嵌杯病毒中国株的特性,采用高通量测序方法测定青岛某养殖场检测的水貂嵌杯病毒的基因组,并对其进行特性分析与比较基因组研究。结果显示,该病毒全基因大小为8 427bp,具有与其他嵌杯病毒相似的结构和基因顺序,5′端具有帽子结构,3′端具有A尾,其与19株代表性毒株的全基因组和衣壳蛋白氨基酸序列同源性分析都证实该病毒与我国2011年报道的水貂嵌杯病毒同源性高,BLAST进一步确认该病毒不属水貂肠道嵌杯病毒,分析结果也对了解嵌杯病毒中各种未分属病毒的分类提供参考。

To analyze the characterization of the mink calicivirus in China,the genome of the mink calicivirus detected in the farm of Qingdao was sequenced by high-throughput sequencing.The genome of the isolate was analyzed and compared with other caliciviruses.The results showed that the length of the isolate was 8 427 bp,which possessed the similar genomic organization,structure and gene order with the other calicivirus.It possessed 5′-untranslated region and 3′-poly（A）tail.The comparing analysis of the genome and capsid protein amino acid sequence of the virus and the 19 representative viruses showed that the identity between this virus and the Chinese virus detected in 2011 was high.The BLAST results showed that this virus was not belong to mink enteric calicivirus.The analysis was also useful to supply reference to the classification of the caliciviruses which haven’t been defied into genera.