肝细胞癌组织中DCLK1蛋白的表达及其临床意义

Expression and clinical significance of DCLK1 protein in tissues of hepatocellular carcinoma

目的探讨肝细胞癌(肝癌)组织中双肾上腺素样激酶1(Doublecortin-like kinase 1,DCLK1)的表达情况、病人的临床病理特点及其与预后的关系。方法收集2012年6月至2015年6月在福建医科大学孟超肝胆医院收治的施行根治性肝癌切除术52例病人的临床病理资料,使用相应癌和癌旁组织构建组织芯片,采用免疫组织化学法检测癌和癌旁组织标本中DCLK1和OV6的表达情况,回顾性分析差异化表达DCLK1病人的性别、年龄、肝硬化状况、甲胎蛋白(AFP)、肿瘤直径、肿瘤数目、包膜状况、微血管侵犯等临床病理指标,Kamplan-Meier法进行生存分析。结果 DCLK1在肝癌组织中高表达18例(34.6%),在癌旁组织中高表达8例(15.4%),结果显示差异有统计学意义(P<0.05);OV6在肝癌组织中高表达20例(38.5%),在癌旁组织中高表达9例(17.3%);DCLK1蛋白在肝癌组织中的表达与AFP、微血管侵犯相关(P值分别为0.007、0.038),且与OV6的表达呈正相关(r=0.505,P<0.001);DCLK1表达水平与肝癌术后3年的无瘤生存率相关(P=0.022)。结论 DCLK1在肝癌组织中表达明显高于癌旁组织,其高表达提示预后不良,DCLK1可能是肝癌诊断及判断预后的一个潜在的分子标志物。

Objective To explore the expression of Doublecortin-like kinase1（DCLK1）in hepatocellular carcinoma（HCC）,and to analyze its correlation with pathologic feature and prognosis.Methods Fifty-two cases of HCC who underwent radical resection of primary liver cancer at the Mengchao Hepatobiliary Hospital of Fujian Medical University were collected and micro arrayed.The expression of DCLK1 and OV6 was detected using immunohistochemistry.The clinicopathologic factors influencing disease-free survival time,age,cirrhosis,AFP,tumor diameter,tumor number,capsule integrity,and microvascular invasion were analyzed.Survival analysis was performed with Kaplan-Meier method.Results DCLK1 expression was increased in 18 cases of HCC（34.6%）,and 8 cases of adjacent tissues（15.4%）（P〈0.05）.OV6 expression was increased in 20 cases of HCC（38.5%）,and 9 cases of adjacent tissues（17.3%）.DCLK1 expression was closely correlated to AFP level（P=0.007）,microvascular invasion（P=0.038）,and positively correlated to the expression of OV6（r=0.505,P〈0.001）.There was significant correlation between 3-year free-survival rate and high expression of DCLK1（P=0.022）.Conclusions DCLK1 expression was increased in HCC.DCLK1 may be a diagnostic and prognostic factor in HCC patients.