湿热环境对CCl_4诱导大鼠肝炎模型的影响及代谢组学研究

The Effects and Metabolomics Study of Damp-Heat Environment on CCl_4-hepatitis Animal Model

目的:从一般体征、生化指标、病理组织、代谢物四个方面考察湿热环境对四氯化碳致大鼠慢性肝炎疾病的影响作用。方法:24只雄性Wistar大鼠随机分为正常组、模型组一(单纯CCl_4,6周)、模型组二(CCl_4+湿热环境,6周)。观察大鼠的体重、饮食量、饮水量等变化,对12项指标进行检测,结合HE染色、Masson染色结果观察肝脏组织受损情况。采用代谢组学技术对血清、肝脏进行差异代谢物鉴定及通路分析。结果:一般体征、生化指标、病理切片结果说明湿热环境加速疾病的进展以及加重疾病严重程度。代谢组学结果显示这种影响涉及血清中氨基酸代谢通路,肝脏中脂质代谢、氨基酸代谢等通路。结论:湿热环境加速疾病的进展以及加重疾病严重程度,该影响机制涉及血清中氨基酸代谢通路,肝脏中脂质代谢、氨基酸代谢等通路。

This study was aimed to investigate the effects of damp-heat environment on chronic hepatitis model by carbon tetrachloride from general signs,biochemical indexes,histopathology and metabolomics.Twenty-four male Wistar rats were randomized into the control group（healthy）,the first model group（CCl4,six weeks）,the second model group（CCl4 with damp-heat environment,six weeks）.Observations were made on the weight,feed intake and drinking amount.Twelve biochemical indexes were detected.HE staining and masson staining results were combined to evaluate liver damage.Metabolomics techniques were used to identify different metabolites and pathways of serum and liver.General signs,biochemical indicators and histopathology showed that damp-heat environment could accelerate disease progress and increase disease severity.Metabolomics results showed that the effects may involve amino acid metabolism in the serum,lipid metabolism,amino acid metabolism and other pathways in the liver.It was concluded that damp-heat environment accelerated disease progression and aggravated disease severity,which involved amino acid metabolism in serum,lipid metabolism,amino acid metabolism and other pathways in the liver.