前列腺癌组织GRP78和GRIM-1表达及其相关性分析

Expression and correlation between GRP78 and GRIM-1 in prostate cancer tissues

目的:探讨前列腺癌组织葡萄糖调节蛋白78(GRP78)和细胞凋亡相关基因-1(GRIM-1)表达及其与预后的关系。方法:采用免疫组织化学染色检测80例前列腺癌和50例良性前列腺增生组织GRP78、GRIM-1表达,比较GRP78、GRIM-1与前列腺癌患者临床资料的关系;Kaplan-Meier检验分析不同GRP78、GRIM-1表达与前列腺癌患者总体生存率的关系,Cox比例风险模型分析影响患者预后的因素。结果:前列腺癌组GRIM-1蛋白阳性率(90.0%,45/50)明显低于前列腺增生组(67.5%,54/80)(χ2=8.578,P=0.003);前列腺癌组GRP78蛋白阳性率(95.0%,76/80)明显高于前列腺增生组(76.0%,38/50)(χ2=10.292,P=0.001)。Gleason 2~7分组GRIM-1蛋白阳性率(82.4%,28/34)明显高于Gleason 8~10分组(56.5%,26/46);Gleason 2~7分组GRP78蛋白阳性率(88.2%,30/34)明显低于Gleason 8~10分组(100.0%,46/46),差异均具有统计学意义(P<0.05)。前列腺癌组织GRIM-1和GRP78表达呈负相关(r=-0.571,P<0.001);GRIM-1高表达组总体生存率明显高于低表达组;GRP78高表达组总体生存率明显低于低表达组,差异具有统计学意义(P<0.05)。多因素分析显示Gleason评分、TNM分期、病理分级、淋巴结转移、GRIM-1和GRP78蛋白表达是影响前列腺癌患者预后的独立因素(均P<0.05)。结论:前列腺癌组织中GRIM-1、GRP78异常表达;GRIM-1低表达和GRP78高表达与前列腺癌患者不良预后有关。

Objective：To explore the expression of glucose-regulated protein 78（GRP78）and gene associated with retinoid and interferon-induced mortality 1（GRIM-1）in prostate cancer tissues and its clinical value for prognositic.Methods：The expression of GRP78 and GRIM-1 in eighty prostate cancer tissues and fifty benign prostatic hyperplasia tissues were performed with immunohistochemical staining,the relationship between GRP78,GRIM-1 and prostate cancer patients clinical data were compared,the over-all survival of patients were analyzed by Kaplan-Meier method,the risk factors influencing the prognosis of prostate cancer patients were analyzed by Cox proportional hazards model.Results：The positive rate of GRIM-1 protein in the prostate cancer group（90.0%,45/50）was significantly lower than that in the benign prostatic hyperplasia group（67.5%,54/80）（χ2= 8.578,P = 0.003）.The positive rate of GRP78 protein in the prostate cancer group（95.0%,76/80）was significantly higher than that in the benign prostatic hyperplasia group（76.0%,38/50）（χ2= 10.292,P = 0.001）.The positive rate of GRIM-1 protein in the 2 ~ 7 score group（82.4%,28/34）was significantly higher than in that in the 8 ~ 10 score group（56.5%,26/46）,while GRP78 positive rate in the 2 ~ 7 score group（88.2%,30/34）was significantly higher than in the 8 ~ 10 score group（100.0%,46/46）,the difference was statistically significant（P 〈 0.05）.There was a negative correlation between GRIM-1 and GRP78 in the prostate cancer tissues（r =-0.571,P 〈 0.001）.The over-all survival in the GRIM-1 higher expression group was markedly higher than that in the lower expression group,and the over-all survival in the higher GRP78 expression group was markedly lower than that in the lower expression group,the difference was statistically significant（P 〈 0.05）.Multivariate analysis showed that Gleason score,TNM stage,pathological grade,lymph node metastasis,GRP78 and GRIM-1 expression were independent risk factors for the poor prognostic of prostate cancer（all P 〈 0.05）.Conclusion：Aberrant expression of GRP78 and GRIM-1 in prostate cancer tissues,the up-regulated of GRP78 and down-regulated of GRIM-1 are risk factors for the poor prognostic of prostate cancer.