ELISA方法检测老年人梅毒螺旋体抗体假阳性原因分析

Analysis on the reasons of false positive of Treponema pallidum antibody in elderly people by ELISA method

目的探索ELISA方法检测梅毒螺旋体抗体阳性情况及其假阳性原因分析。方法选取2015年2月至2017年11月来我科就诊的患者作为研究对象,根据年龄将受检者分为老年组和对照组,并采用ELISA法检测两组患者血清中的梅毒螺旋体抗体,检测阳性者再次采用梅毒螺旋体明胶颗粒凝集试验(TPPA)作为确诊方法进行对比分析。结果 5216例受检者中,TP-ELISA法检测阳性者212例,阳性率为4.06%,且老年组阳性率(5.04%)高于对照组(3.25%);经TPPA法确诊阳性者143例,假阳性率为1.32%,且老年组假阳性率(1.97%)高于对照组(0.78%)。结论年龄可造成ELISA法检测梅毒螺旋体抗体出现假阳性。

Objective Exploring the situation of false positive of ELISA method in detecting Treponema pallidum antibody, and analyzing the reasons for false positive.Methods We selected the patients who visited our department during February 2015 and November 2017 as the subjects. All the subjects were divided into two groups （the aged group and the control group） according to the age, and detected the antibody of Treponema pallidum in the serum by ELISA method. The positive people were made a definite diagnose using the Treponema pallidUm gelatin particle agglutination test （TPPA） once more. Results Among all the 5216 subjects, 212 subjects were detected positive for TP-ELISA test, and the positive rate was 4.06%, and the positive rate in the elderly group （5.04%） was higher than that in the control group （3.25%）. Herein, 143 subjects were confirmed by TPPA method, and the false positive rate was 1.32%, while the false positive rate in the elderly group （ 1. 97%） was higher than that in the control group （0. 78%） once more. Conclusions Age can result in false positive detection of Treponema pallidum antibody by ELISA method.