参景固本方对D-半乳糖致亚急性衰老模型小鼠的抗氧化作用

Study on antioxidant effect of Shenjing Guben prescription on D-galactose-induced aging model

目的研究参景固本方对D-半乳糖致亚急性衰老模型小鼠的影响。方法将70只小鼠随机分为7组,即正常对照组,模型组,参景固本方低、中、高剂量组,金匮肾气丸组及维生素E组,每组10只,除正常对照组外全部小鼠按125 mg/kg剂量颈背部皮下注射D-半乳糖建立衰老模型。以参景固本方与金匮肾气丸和维生素E对比治疗,观察其学习记忆能力,测定增重率、胸腺指数及脾脏指数,测定脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)等指标。结果参景固本方高、中、低剂量组平均寻台时间显著降低(P<0.01);参景固本方高剂量组增重率降低(P<0.01);参景固本方高剂量组胸腺指数及脾脏指数明显增高(P<0.01),参景固本方中剂量组脾脏指数略增高(P<0.05);参景固本方高剂量组MDA含量显著降低(P<0.01),SOD、T-AOC活性显著升高(P<0.01),参景固本方中剂量组SOD活性略有升高(P<0.05)。结论参景固本方能够改善D-半乳糖致衰老模型小鼠的学习记忆能力,提高脑组织的抗氧化能力,具有一定延缓脑衰老的作用。

Objective To study the effect of Shenjing guben on subacute aging model mice.Methods Seventy mice were randomly divided into 7 groups：normal control group,model group,low,middle and high dose of Gubenfang group,Jingui Shenqiwan group and vitamin E group,with 10 in each group mice（except for the normal control group）were dosed subcutaneously with D-galactose at the dose of 125 mg/kg to establish the aging model.To observe the Guben side and the Jingui Shenqi pill and VE contrast treatment,observe the learning and memory ability,weight gain rate,thymus index and spleen index,determination of brain tissue MDA,SOD and T-AOC and other indicators.Results The weight gain rate of Shen Jing Guben high-dose group decreased（P〈0.01）.The expression of thymus index and spleen（P〈0.01）.The spleen index of Shen Jing Guben recipe group was slightly increased（P〈0.05）,while the activity of SOD and T-AOC of Shenjing Guban high dose group was significantly increased（P〈0.01）,MDA content was significantly lower（P〈0.01）,and Shenjing Guben middle dose group SOD activity increased slightly（P〈0.05）.Conclusion Shenjing Guben prescription can improve the learning and memory ability of aging mice and enhance the anti-oxidative capacity of mouse brain tissue to achieve the purpose of anti-aging.