离子对试剂对离子对色谱-间接紫外检测分析哌啶离子液体阳离子的影响

Effect of Ion-pair Reagent on Analysis of Piperidinium Ionic Liquid Cations by Ion-pair Chromatography with Indirect Ultraviolet Detection

本文研究了不同的离子对试剂对离子对色谱-间接紫外检测测定哌啶离子液体阳离子的影响,探讨了保留和检测规律。研究的离子对试剂有戊烷磺酸钠、庚烷磺酸钠、辛烷磺酸钠和癸烷磺酸钠。结果表明,离子对试剂的烷基链越长,哌啶阳离子的保留时间越长,检出限总体趋势提高;流动相中有机溶剂甲醇含量增加,哌啶阳离子的保留时间缩短,检出限降低。哌啶阳离子的保留机理主要是动态离子交换机理。用庚烷磺酸钠和辛烷磺酸钠作为离子对试剂可使哌啶离子液体阳离子获得较好的分离分析。

This paper is focused on the effects of the ion-pair reagents on determination of piperidinium ionic liquid cations by ion-pair chromatography with indirect ultraviolet detection, and discusses the rules of retention and detection. Ion-pair reagents used in the study were sodium 1-pentanesulfonate, sodium 1- heptanesulfonate,sodium 1-octanesulfonate and sodium 1-decanesulfonate. The results show that with the increase of the lengths of the alkyl chain of ion-pair reagent, the retention time is longer, the detection limit increases overall trend; the higher the methanol content in the mobile phase is, the shorter the retention time is, and the lower the detection limit is. The retention mechanism of the piperidinium cations is mainly the dynamic ion exchange mechanism. Experimentally, using sodium 1-heptanesulfonate and sodium 1-octanesulfonate as ion-pair reagent is a suitable choice for separation and detection of piperidinium cations.