马桑水提取物对大鼠深Ⅱ度烧伤创面微循环和氧化应激的影响

Effects of Coriaria Sinica Maxim's extract on microcirculation and oxidative stress of wounds in rats with deep second-degree burn

目的:探索马桑水提取物(CSME)对大鼠深Ⅱ度烧伤创面微循环和氧化应激的影响。方法:随机将180只SD大鼠分成6组(n=30):对照组(生理盐水组,NS)、凡士林组(WPL)、磺胺嘧啶银组(SSD)、马桑提取物组(低剂量组(CSME-L)、中剂量组(CSME-M)和高剂量组(CSME-H)。麻醉后用烫伤仪烫伤脱毛区(病理结果证实深Ⅱ度烫伤),伤后创面分别涂擦NS、WPL、SSD和不同浓度的CSME烧伤软膏。于伤后48 h、7 d、14 d、21 d计算创面愈合率(HR),检测创面微血管(MVD)、创面组织含水量(TM)、血管内皮生长因子(VEGF)、丙二醛(MDA)、超氧化物歧化酶(SOD)、羟脯氨酸(HYP),进行创面组织学观察。结果:CSME各组的HR呈剂量依赖性大于NS和WPL(P<0.05);在21 d,其创面组织血管管径、数量、分布及TM呈剂量依赖性少于其他各组(P<0.05),伤后7 d、14 d,CSME各组呈现剂量依赖性高于NS、WPL和SSD(P<0.05),而伤后21 d呈剂量依赖性低于NS、WPL和SSD(P<0.05);CSME的SOD活性、HYP、NO和ET含量呈剂量依赖性高于其他各组(P<0.05),而MDA活性呈剂量依赖性弱于其他各组(P<0.05),CSME呈剂量依赖性缩短创面修复时间,瘢痕少于其他各组。结论:CSME具有早期减轻烧伤创面组织水肿、促进创面收缩、加快创面结痂以及促进肉芽组织增生,有利于创面愈合;后期则抑制创面肉芽组织增生,防止瘢痕过度增生。其机制与创面微循环、氧化应激、NO和VEGF的调控有关。

Objective： To investigate the effects of Coriaria Sinica Maxim’s extract（ CSME） on microcirculation and oxidative stress of wounds in rats with deep second-degree burn. Methods： One hundred and eighty rats were randomly divided into normal saline group（ NS）,white petroleum group（ WPL）,silver sulfadiazine group（ SSD）,Coriariasinica Maxim’s extract group which were divided into low dose（ CSME-L）,middle dose（ CSME-M） and high dose（ CSME-H）. After anesthesia with burn instrument to burn the hair removal area of rats,these wounds were confirmed by pathological results with deep second degree burns.And then,those drugs were applied respectively on the wounds,such as NS、WPL、SSD and different concentrations of CSME. After injury at 48 h,7 d,14 d and 21 d,the healing rate（ HR） of wound was measured,and the microvessel density（ MVD）,tissue moisture（ TM）,vascular endothelial growth factor（ VEGF）,model driven architecture（ MDA）,superoxide dismutase（ SOD） and hydroxyproline（ HYP） were detected,too. All pathological sections of the wound tissue were observed. Results： The HR of CSME groups were obviously increased with a dose-dependent manner,which was significantly higher than that of NS and WPL（ P〈0.05）; On the 21 stday,the diameter,number,distribution of the vessels and and the TM were less than other groups with a dose-dependent manner; On the 7 thand 14 thday after injury,CSME groups were significantly higher than the NS,WPL and SSD with a dose-dependent manner（ P〈0.05）,but,on the 21 stday after injury,they were lower than NS,WPL and SSD with a dose-dependent（ P〈0.05） manner. The levels of SOD,HYP,NO and ET in CSME groups were higher than those in other groups with dose-dependent on SOD activity,HYP,NO and ET content（ P〈0.05）,while MDA activity was weaker than other groups（ P〈0.05）. Similarly,pathological findings were also shown that CSME groups were better than other groups with a dose-dependent manner in decrease decreasing of wound repair time and hyperplasia of scar tissue. Conclusion： CSME can relieve tissue edema,promote wound contraction,speed up the formation of eschar and accelerate the proliferation of granulation tissue,which are beneficial to the wound healing in the early stages. But,it can inhibit the hyperplasia of granulation tissue to prevent the excessive scar hyperplasia of burn wound in the later stages. Its mechanism is related to regulation what microcirculation,oxidativestress,NO and VEGF.