电针对动脉粥样硬化兔腹腔巨噬细胞中清道夫受体AⅠ表达的影响

Effect of Electroacupuncture on Expression of Scavenger Receptor A Ⅰin Peritoneal Macrophages of Atherosclerotic Rabbits

目的:观察电针对动脉粥样硬化(AS)兔腹腔巨噬细胞中清道夫受体AⅠ(SR-AⅠ)蛋白和mRNA表达的影响,探讨电针抗AS的作用机制。方法:将新西兰兔随机分为空白组、模型组、电针组、西药组,每组6只。高脂饲料喂养结合颈总动脉球囊损伤术建立AS模型。电针组电针"足三里""内关""关元"穴(1mA,2Hz,20min,1次/d),西药组予阿托伐他汀钙片灌胃(1mg·kg-1·d-1),均治疗4周。HE染色法观察兔术侧颈总动脉病理形态;Western blot法检测兔腹腔巨噬细胞中SR-AⅠ蛋白的表达;PCR技术检测SR-AⅠmRNA的表达。结果:HE染色可见AS兔术侧颈总动脉管内有粥样斑块形成;与模型组相比,电针组和西药组兔颈总动脉粥样硬化组织损伤明显减轻。与空白组比较,模型组兔腹腔巨噬细胞中SR-AⅠ蛋白及mRNA表达水平显著升高(P<0.01);与模型组比较,电针组和西药组兔腹腔巨噬细胞中SR-AⅠ蛋白及mRNA表达水平显著降低(P<0.01);电针组与西药组相比各指标的差异均无统计学意义(P>0.05)。结论:电针可抑制AS兔腹腔巨噬细胞中SR-AⅠ蛋白和mRNA的表达,这可能是针刺防治AS的新机制。

Objective To observe the effect of electroacupuncture（EA）on the expression of scavenger receptor A Ⅰ（SR-A Ⅰ）protein and mRNA in peritoneal macrophages of atherosclerosis（AS）rabbits.Methods A total of 26 New Zealand rabbits were used in the present study,among them,7 were randomly selected to constitute a blank control group,and the rest 19 rabbits were fed with high-fat diet combined with carotid artery balloon injury for establishing AS model.Eight weeks after high-fat forage feeding,one high-fat fed rabbit and one normal rabbit were randomly selected for verifying the success of modeling.HE staining was employed to examine pathological changes of the common carotid artery after paraffin section.Then the rest 18 rabbits were randomly divided into model,EA and medication groups（n= 6 rabbits in each）.EA（2 Hz,1 mA）was applied at＂Guanyuan＂（ST 25）and bilateral＂Zusanli＂（ST 36）and＂Neiguan＂（PC 6）for 20 min.The rabbits of the medication group were treated by gavage of Atorvastatin Calcium（1 mg·kg^-1·d^-1）,and those of the model group treated by gavage of distilled water（2 mL·kg^-1·d^-1）.The treatment was given once daily for 4 weeks,with one day＇s interval between every two weeks.Three days before termination of the experiments,sterile starch solution（3-4 mL/animal）was injected into the peritoneal cavity,and at the end of the experiment,the peritoneal fluid was collected and centrifugated to be cultivated in Dulbecco＇s Modified Eagle Medium（DMEM）for collecting the macrophages.The expression of SR-A Ⅰ protein and mRNA in macrophages was assayed by Western blot and PCR,respectively.Results HE staining displayed an injury of the inner membrane of the carotid artery marked by observable atherosclerotic plaques,interruption,thickening and uplift,inflammatory cell infiltration,etc.in the AS model rabbits which were relatively milder in both EA and medication groups.The expression levels of SR-AⅠ protein and mRNA were significantly up-regulated in the model group relevant to the blank control group（P〈0.01）,and considerably down-regulated in both EA and medication groups compared with the model group（P〈0.01）.There were no statistical differences between the EA and medication groups in the decreased levels of expression of SR-AⅠ protein and mRNA（P〉0.05）.Conclusion EA intervention may improve the severity of atherosclerosis to a certain degree in AS rabbits,which is possibly associated with its effect in inhibiting the expression of SR-A Ⅰ protein and mRNA in the peritoneal macrophages.