高糖环境对骨髓间充质干细胞增殖的影响

Effect of high glucose on proliferation of bone marrow mesenchymal stem cells

目的 研究不同浓度葡萄糖对骨髓间充质干细胞（BMMSCs）增殖的影响。 方法 将传代至第4～6代的BMMSCs接种培养，对照组采用DMEM/F12（1∶1）完全培养基（基础葡萄糖浓度为5.5 mmol/L）；实验组采用含不同浓度葡萄糖（20.5、25.5、30.5、35.5 mmol/L）的DMEM/F12（1∶1）高糖培养基，连续培养 15 d。采用噻唑蓝（MTT）比色法分别检测每天各浓度组的吸光度（A）值。 结果 与对照组相比，20.5 mmol/L组和30.5 mmol/L组第2、3、5天的A值升高，差异均具有统计学意义（均P〈0.05）；25.5 mmol/L组第2、3、5、8天的A值升高，差异均具有统计学意义（均P〈0.05）；35.5 mmol/L组第2、3、5、8天的A值升高，第4天的A值降低，差异均具有统计学意义（均P〈0.05）。将20.5 mmol/L组、25.5 mmol/L组、30.5 mmol/L组和35.5 mmol/L组进一步两两比较，结果显示：35.5 mmol/L组第4、12天的A值低于20.5 mmol/L组，差异均具有统计学意义（均P〈0.05）；30.5 mmol/L组和35.5 mmol/L组第11、14天的A值低于25.5 mmol/L组，差异均具有统计学意义（均P〈0.05）。 结论 高糖环境能在短期内促进BMMSCs增殖，但随着培养时间的延长，促进细胞增殖能力逐渐减弱，较高浓度葡萄糖的高糖环境会对细胞增殖产生抑制作用。

Objective To study the effects of concentration of glucose on the proliferation of bone marrow mesenchymal stem cells（BMMSCs）.Methods BMMSCs passaged to the 4th to 6th were inoculated and cultured.The control group was treated with DMEM/F12（1∶1）standard medium,and the basal glucose concentration was 5.5 mmol/L.The experimental group was treated with DMEM/F12（1∶1）high-glucose medium with the concentration of 20.5,25.5,30.5 and 35.5 mmol/L respectively,and continuously cultured for 15 d.The absorbance（A）values of each group once per day were measured by thiazolyl blue（MTT）colorimetry.Results Compared with the control group,the absorbance was increased at the 2nd,3rd,and 5th days in the 20.5 and 30.5 mmol/L groups,and the differences were statistically significant（all P〈0.05）.For the 25.5 mmol/L group,the absorbance was increased at the 2nd,3rd,5th and 8th days,and the differences were statistically significant（all P〈0.05）.For the 35.5 mmol/L group,the absorbance was increased at the 2nd,3rd,5th and 8th days and decreased at the 8th day,and the differences were statistically significant（all P〈0.05）.The results of 20.5,25.5,30.5 and 35.5 mmol/L groups were further compared one another.The results showed that the absorbance at the 4th and 12th days in the 35.5 mmol/L group were lower than that of the 20.5 mmol/L group,and the differences were statistically significant（all P〈0.05）.The absorbance at the 11th and 14th days in the 30.5 and 35.5 mmol/L groups were lower than that of the 20.5 mmol/L group,and the differences were statistically significant（all P〈0.05）.Conclusions High-glucose environment can promote the proliferation of BMMSCs in a short time.However,with the prolongation of culture time,this effect is gradually weakened because of the inhibiting effect of high-glucose environment to the cell proliferation.