多肽菌素对肉鸡血清生化指标、血清抗体水平及免疫器官指数的影响

Effects of Polypeptide on Serum Biochemical Index,Serum Antibody Level and Immune Organ Index of Broilers

试验旨在研究多肽菌素对肉鸡血清生化指标、血清抗体水平及免疫器官指数的影响,以验证多肽菌素提高肉鸡机体免疫力的能力。4万只1日龄科宝500肉雏鸡随机分为2组,即试验组和对照组,每组4个重复,每个重复5 000只。对照组按原有免疫和用药程序7日龄、14日龄及21日龄均进行新城疫和禽流感疫苗免疫;试验组鸡只在7日龄时进行新城疫和禽流感疫苗免疫,并在13~15日龄饲喂添加多肽菌素(400 g/t)的日粮,20~22日龄饲喂添加多肽菌素(300 g/t)的日粮。试验期28 d。结果显示:试验组28日龄肉鸡血清总蛋白含量、球蛋白含量、球蛋白与白蛋白比值(球/白)分别比对照组提高10.75%、15.17%、11.45%,谷草转氨酶活性比对照组提高6.23%,差异显著(P<0.05);试验组肉鸡新城疫病毒抗体效价比对照组提高19.89%,差异显著(P<0.05),禽流感病毒抗体效价比对照组提高6.97%,差异显著(P<0.05);试验组肉鸡脾脏指数比对照组提高17.05%,差异显著(P<0.05),试验组胸腺指数比对照组提高11.89%,差异显著(P<0.05)。研究表明:肉鸡日粮添加多肽菌素,可以显著改善血清生化指标,提高机体抗体水平及免疫器官指数。

This experiment was conducted to study the effects of polypeptide on serum biochemical index, antibody level and immune organ index of broilers in order to verify the ability of polypeptide in improving broilers′ immunity ability. A total of 40 000 one-day-age Cobb500 chicks were randomly allotted to two treatments with four replicate per treatment and 5 000 chicks per replicate. Broilers in the control group were inoculated Newcastle disease vaccine and H9 subtype avian influenza vaccine according to the original immunization and pharmacy program at 7, 14 and 21 days of age, respectively. Broilers in experimental group were inoculated the above vaccines at 7 days of age and fed with basal diet with 400 g/t polypeptide at 13 to 15 days of age and 300 g/t polypeptide at 20 to 22 days of age. The trial lasted for 28 days. The results showed that serum total protein, globulin content and the ratio of globulin and albumin in experiment group were increased significantly by 10.75%, 15.17%, 11.45%. Meanwhile, serum glutamic oxalacetic transaminase activity in experiment group was improved by 6.23%（P〈0.05）. The antibody titers against Newcastle disease virus and H9 subtype avian influenza virus in experiment group were increased significantly by 19.89% and 6.97%than those of the control group（P〈0.05）, respectively. And meanwhile the spleen index and the thymus index in experiment group were improved obviously by 17.05% and 11.89% compared with the control group（P〈0.05）, respectively.It′s concluded that dietary polypeptide could improve serum biochemical index, antibody level and immune organ index of broilers.