阿散酸磁性分子印迹聚合物的制备及其应用

Preparation and Application of Arsanilic Acid Magnetic Molecularly Imprinted Polymers

以阿散酸(ASA)为模板分子,采用紫外-可见吸收光谱法选择2-乙烯吡啶(2-VP)为功能单体,以改性Fe_3O_4@SiO_2为载体,通过表面印迹法合成阿散酸磁性分子印迹聚合物(MIP)。采用红外光谱法对化合物的结构和组成进行表征,并采用静态、动态和选择性吸附试验对其性能进行研究。结果表明:MIP对阿散酸具有良好的吸附性能,其最大吸附量为5.17g·kg^(^(-1))。以MIP为固相萃取填料,结合高效液相色谱法,对鸡肝中阿散酸进行分离、富集和测定,其检出限(3S/N)为1.10×10^(-3) mg·kg^(^(-1))。按标准加入法进行回收试验,回收率为82.1%~94.7%,测定值的平均相对标准偏差(n=5)为3.4%。

Arsanilic acid magnetic molecularly imprinted polymer（MIP） was synthesized by surface polymerization technique using arsanilic acid（ASA）as template molecule,2-vinyl pyridine（2-VP）as functional monomer selected by UV-vis absorption spectra and denatured Fe3O4 @SiO2 as the carrier.The structure and composition of the compound were characterized by infrared spectroscopy,and the properties of the compound were studied by static,dynamic and selective adsorption tests.As shown by the results,MIP had good adsorption properties for ASA,and its maximum quantity of adsorption was 5.17 g·kg^-1.ASA in chicken liver was separated,enriched and determined using MIP as solid phase extraction packing,combined with high performance liquid chromatography.Detection limit（3 S/N）of the method was 1.10×10^-3 mg·kg^-1,and test for recovery was made by standard addition method,giving results in the range of 82.1%-94.7%,with average RSD（n=5）of3.4%.