白酒酒糟中高温菌的降解特性研究

Study on Degradation Characteristics of High Temperature

研究白酒酒糟中分离到的高温菌对有机物的降解特性。分别在淀粉降解培养基、纤维素降解培养基和蛋白质降解培养基中接种7株高温菌菌株,培养后通过观察和测定透明圈的大小来了解各菌株对着三大类物质的降解特性。菌株DX有很强的蛋白质分解能力,同时淀粉分解能力也较强,其H_c值为2.80;菌株XX淀粉分解能力很强,同时具有一定的纤维素分解能力;菌株DF具有较强的纤维素分解能力,其H_c值能达到3.01,同时具有一定的淀粉和蛋白质分解能力;菌株M4有一定的淀粉和蛋白质分解能力;菌株M5纤维素分解能力较强,其H_c值能达到3.21,有一定的淀粉分解能力;菌株M1,M6有一定的淀粉、纤维素和蛋白质分解能力。通过了解各菌株的降解特性,可为下阶段降解酒糟的复合菌剂的制备提供参考和依据。

The degradation characteristics of seven kinds of thermophilic bacteria in vinasse of liquor were studied. The thermophilic bacteria was incubated in the starch degradation medium, the cellulose degradation medium, and the protein degradation medium respectively. And the sizes of the transparent circles on these media were observed and measured after a period of incubation time, in order to distinguish the degradation characteristics of these thermophilic bacteria to starch, cellulose, and protein. The results showed that at 55 ℃, strain DX, whose H_c value was 2.80, has strong abilities of proteolysis and starch decomposing; Strain XX had a strong ability of starch decomposing, but also had a certain ability of cellulose decomposing; Strain DF, which its H_c value could reach 3.01, has a strong ability of cellulose decomposing, and has certain abilities of starch and protein decomposing; strain M4 had certain abilities of starch and protein decomposing; strain M5, whose H_c value could reach 3.21, has a strong ability of cellulose decomposing, and a certain ability of starch decomposing; Strain M1 and strain M6 had certain abilities of starch, cellulose and protein decomposing. Understanding the degradation characteristics of these thermophilic bacteria could provide references and evidences of the complex microbial inoculants preparation in further study.