碳源和乙醇调控雨生红球藻生长与虾青素积累

Different carbon sources plus ethanol improve the growth rate and astaxanthin accumulation in Haematococcus pluvialis

[目的]雨生红球藻(Haematococcus pluvialis)生长速率和虾青素积累量决定着雨生红球藻商业化生产的经济效益。本文旨在建立提高雨生红球藻生长和虾青素积累的新方法。[方法]以BG-11为基础培养基,分别以乙酸钠和二氧化碳为碳源,添加少量乙醇,探讨乙醇和碳源对雨生红球藻生长及虾青素积累的效应。[结果]添加乙醇显著提高了雨生红球藻生长及虾青素积累。(BG-11+乙酸钠)+乙醇培养的雨生红球藻生长迅速,在第4天细胞密度上升到1.587×106 cells·mL^(-1),而对照组(BG-11+乙酸钠)在第8天细胞密度才上升到1.187×106 cells·mL^(-1)。(BG-11+乙酸钠)+乙醇培养的雨生红球藻在第14天生物量和虾青素含量分别达2.74g·L-1和3.51%(干重),分别是对照组的2.98倍和1.71倍,显著高于对照组。同样,(BG-11+CO2)+乙醇培养的雨生红球藻在第6天细胞密度上升到1.236×106cells·mL^(-1),在第14天生物量和虾青素含量分别达2.59g·L-1和3.1%(干重),均高于对照组(BG-11+CO2)的生物量(0.7g·L-1)和虾青素含量(1.43%,干重)。[结论]研究结果为建立基于添加乙醇提高雨生红球藻生长速率和虾青素积累量的简单易行规模化技术体系提供了科学参考。

[Objective]The growth rate and astaxanthin content in Haematococcus pluvialis determine the economic efficiency of commercial products.The current study was conducted to develop a new method to enhance the growth rate and astaxanthin biosynthesis in H.pluvialis.[Methods]In this study,BG-11 was used as basic medium.Gaseous carbon dioxide（CO2）and sodium acetate were selected as two carbon sources,with or without ethanol as different treatments.The effects of ethanol and carbon sources on the growth and astaxanthin biosynthesis of H.pluvialis were measured at certain time interval.[Results]Results indicated that the growth rate and astaxanthin biosynthesis in H.pluvialis were significantly increased with ethanol addition（P0.05）H.pluvialis cultured in the medium of（BG-11＋sodium acetate）＋ethanol grew fast,and cell density was quickly reached up to the highest of 1.587×106 cells·mL^（-1） after 4 days treatment,while algae in the control group（BG-11＋sodium acetate）grew slowly,and the highest cell density（1.187×106 cells·mL^（-1））was reached on 8 days post treatment.Cell biomass and astaxanthin content in the treatment of（BG-11＋sodium acetate）＋ethanol were increased to 2.74 g·L-1 and 3.51 %（dry weight,DW）,which were significantly higher than those in control group（BG-11＋sodium acetate）by 2.98 and 1.71 fold,respectively on 14-days post treatment.Similarly,H.pluvialis cultured in the medium of（BG-11＋CO2）＋ ethanol grew speedy to the greatest cell density of 1.236×106 cells·mL^（-1） on 6 days post treatment,followed by accumulating biomass of 2.59 g·L-1 and astaxanthin content of 3.10%（DW）on 14 days,which were both higher than that in the control group（BG-11＋CO2）at 0.7 g·L-1 and1.43%（DW）,respectively.[Conclusion]The present findings provide the scientific reference for developing a simple and effec-tive system of large-scale cultivation with the addition of environment-friendly and low-cost solvent,ethanol,as a growth regulator to increase the growth rate and astaxanthin accumulation in H.pluvialis.