光合细菌利用有机废水连续产氢的试验研究

Experimental study on continuous production of hydrogen with photosynthetic bacteria from organic wastewater

为考察光合细菌连续性生物制氢过程中碳源浓度、水力停留时间等因素对连续运行稳定性能和制氢量的影响,通过改变碳源浓度和水力停留时间来测定产氢量以及产氢率,确定最佳的连续运行条件。结果表明:光合细菌利用含糖废水为基质进行连续性生物制氢过程中,在最佳温度为30℃、最佳光照强度为3 000 Lux的情况下,且当葡萄糖质量浓度为12 g/L,水力停留时间为72 h时,产氢效率最高,连续产氢速率达45 m L/h。

In order to investigate the effect of carbon source concentration, hydraulic retention time and some other factors on the stability and the hydrogen production capacity of the photosynthetic bacteria continuous hydrogen production system, an experiment was carried out. Through changing the carbon source concentration and the hydraulic retention time to measure hydrogen production capacity and rate, the optimal condition for the system continuous operation was determined. The results of the experiment showed that, for the continuous hy-drogen production by photosynthetic bacteria with sucrose -co n tain in g wastewater as the substrate, the optimal condition was as follows： the temperature was 30 ℃, the illumination intensity was 3 000 Lux, the mass concen-tration of glucose was 12 g/L, the hydraulic retention time was 72 h . Under the above condition, the hydrogen production rate reached the highest and the velocity was as high as 45 mL/h .