外周血淋巴细胞亚群检测对儿童亚急性坏死性淋巴结炎的诊疗意义

Significance of peripheral blood lymphocyte subsets detection in the diagnosis and treatment of children with subacute necrotizing lymphadenitis

目的探讨外周血淋巴细胞亚群检测对儿童亚急性坏死性淋巴结炎(CSNL)的诊疗意义。方法选取2013年1月至2016年12月河南省人民医院小儿外科及内科收治的CSNL患儿51例为CSNL组,同期选择门诊健康体检儿童30例为对照组。采用流式细胞技术检测两组受试对象外周血T淋巴细胞亚群百分率,并观察CSNL治疗效果。结果 51例CSNL患儿中,治愈38例,好转12例,无效1例。治疗前CSNL患儿CD3^+、CD4^+、CD19^+淋巴细胞亚群百分率和CD4^+/CD8^+均显著低于对照组,差异有统计学意义(P<0.05)。38例治愈患儿和12例好转患儿治疗中、后外周血CD3^+、CD4^+、CD19^+淋巴细胞亚群百分率和CD4^+/CD8^+显著高于治疗前,治疗后的指标又显著高于治疗中,差异有统计学意义(P<0.05)。结论细胞亚群CD3^+、CD4^+、CD4^+/CD8^+及CD19^+,可作为临床CSNL诊疗的重要评估指标。

Objective To investigate the significance of peripheral blood lymphocyte subsets detection in the diagnosis and treatment of children with subacute necrotizing lymphadenitis（CSNL）.Methods The percentage of lymphocyte subsets in peripheral blood of 51 children with CSNL（CSNL group）and 30 healthy children（control group）was detected by flow cytometry（FCM）,and the treatment results of CSNL were observed.Results In the 51 cases of CSNL,38 were cured,12 improved and 1 failed.Before treatment,the percentage of CD3^＋,CD4^＋and CD19^＋of lymphocyte subsets and CD4^＋/CD8^＋of CSNL group were significantly lower than control group（P〈0.05）.The percentage of CD3^＋,CD4^＋and CD19^＋of lymphocyte subsets and CD4^＋/CD8^＋of the 51 children with CSNL were significantly higher during and after treatment than before treatment（P〈0.05）,and the index after the treatment was higher than that during treatment（P〈0.05）.Conclusion The CD3^＋,CD4^＋and CD19^＋of peripheral blood lymphocyte subsets and CD4^＋/CD8^＋can be used as important indicators for clinical diagnosis and treatment of CSNL.