HPLC法测中药“回阳液”中人参皂苷Rb1、Rg1、Re的含量研究

Study on determination for content of Rb1, Rg1, Re in ginsenoside of traditional

文章通过建立可同时测定中药复方注射液回阳液中人参皂苷Rb1、Rg1、Re含量的高效液相色谱方法,以便为建立HYY的质量标准提供依据。采用的方法是Agilent Zorbax SB-C_(18)柱(4.6 mm×150 mm,5μm),以乙腈-水为流动相进行梯度洗脱,流速1.0 m L/min,柱温25℃,检测波长203 nm。实验结果显示在本色谱条件下,人参皂苷Rb1、Rg1、Re分离度良好,系统适应性实验表明3种成分的检测其线性关系良好(R^2_(Rg1)=0.999 2;R^2_(Rb1)=0.999 1;R^2_(Re)=0.999 5);其精密度、重复性、稳定性良好;人参皂苷Rb1、Re、Rg1的平均加样回收率分别为97.59%,101.32%和98.87%(RSD值分别为2.12%,1.77%,2.29%),符合要求。实验结果表明,建立的HPLC分析检测方法可以用作复方中药HYY的质量控制。

This paper through establishing an RP-HPLC method for the simultaneous determination of ginsenoside Rb1, Rg1 and Re in compound injection HYY of traditional Chinese medicine, which provided the basis for the establishment of the quality standard of HYY. Using Agilent Zorbax SB-C18 column（4.6 mm×150 mm,5 μm）acetonitrile-water as a mobile phase at 25 ℃.The fow rate was 1.0 mL.min-1 and the detection wavelength was set at 203 nm. Under the chromatographic condition, ginsenoside Rb1, Rg1 and Re all had good separation. The system adaptability experiment showed that ginsenoside Rb1, Rg1 and Re all had a very good linear relationship（R^2Rg1=0.999 2; R^2Rb1=0.999 1; R^2Re=0.999 5）good precision, good repeatability, good stability and high recovery rate of 97.59%, 101.32% and 98.87%.The RSD of three ginsenosides were 2.12%, 1.77% and 2.29%.The HPLC analysis and detection method established in this experiment can be used as the quality control of the compound Chinese medicine HYY.