摘要
BACKGROUND:Early reperfusion can effectively treat acute myocardial infarction(AMI) and reduce the mortality signif icantly. This study aimed to compare the role of plasma microRNA-1(miR-1) and cardiac troponin T(cTnT) in early diagnosis of AMI patients.METHODS:From May 2011 to May 2012,plasma samples were collected from 56 AMI patients and 28 non-AMI controls. The expression of plasma miR-1 was measured by quantitative reverse transcription-polymerase chain reaction(qRT-PCR),and the level of plasma cTnT was measured using electrochemiluminescence-based methods on an Elecsys 2010 Immunoassay Analyzer. SPSS 16.0 was used for the statistical analysis of the results. Data were expressed as mean±standard deviation unless otherwise described. The differences about clinical characteristics between the AMI patients and controls were tested using Student's t test or Fisher's exact test. The Mann-Whitney U test was conducted to compare the expression of microRNAs between the AMI patients and controls. MicroRNAs expression between different intervals of the AMI patients was compared using Wilcoxon's signed-rank test. The receiver operating characteristic(ROC) curve was established to discriminate the AMI patients from the controls.RESULTS:In the present study,the expression of plasma miR-1 was signifi cantly increased in the AMI patients compared with the healthy controls(P<0.01). The plasma miR-1 in the AMI patients decreased to the normal level at 14 days(P>0.05). The expression of plasma miR-1 was not related to the clinical characteristics of the study population(P>0.05). ROC curve analyses demonstrated that miR-1 was specifi c and sensitive for the early diagnosis of AMI,but not superior to cTnT.CONCLUSION:Plasma miR-1 could be used in the early diagnosis of AMI,but it is similar to cTnT.
BACKGROUND： Early reperfusion can effectively treat acute myocardial infarction （AMI） and reduce the mortality significantly. This study aimed to compare the role of plasma microRNA-1 （miR-1） and cardiac troponin T （cTnT） in early diagnosis of AMI patients. METHODS： From May 2011 to May 2012, plasma samples were collected from 56 AMI patients and 28 non-AMI controls. The expression of plasma miR-1 was measured by quantitative reverse transcription-polymerase chain reaction （qRT-PCR）, and the level of plasma cTnT was measured using electrochemiluminescence-based methods on an Elecsys 2010 Immunoassay Analyzer. SPSS 16.0 was used for the statistical analysis of the results. Data were expressed as mean＋standard deviation unless otherwise described. The differences about clinical characteristics between the AMI patients and controls were tested using Student＇s t test or Fisher＇s exact test. The Mann-Whitney U test was conducted to compare the expression of microRNAs between the AMI patients and controls. MicroRNAs expression between different intervals of the AMI patients was compared using Wilcoxon＇s signed-rank test. The receiver operating characteristic （ROC） curve was established to discriminate the AMI patients from the controls. RESULTS： In the present study, the expression of plasma miR-1 was significantly increased in the AMI patients compared with the healthy controls （P〈0.01）. The plasma miR-1 in the AMI patients decreased to the normal level at 14 days （P〉0.05）. The expression of plasma miR-1 was not related to the clinical characteristics of the study population （P〉0.05）. ROC curve analyses demonstrated that miR-1 was specific and sensitive for the early diagnosis ofAMI, but not superior to cTnT. CONCLUSION： Plasma miR-1 could be used in the early diagnosis of AMI, but it is similar to cTnT.
基金
supported by grants from the National Natural Science Foundation of China(81071030)
the Science and Technology Foundation of Guangdong Province(2011B080701006)
