高温条件下桑螟肠道微生物的多样性分析

Diversity analysis on gut microbiota of Glyphodes pyloalis Walker under high temperature

为研究桑螟(Glyphodes pyloalis Walker)抗逆生理与肠道微生物的相关性,通过16S r DNA高通量测序技术对高温处理前后的桑螟肠道微生物进行测序,研究桑螟肠道微生物多样性及在高温下肠道微生物的变化对其抗逆生理的作用。温度处理试验表明:在桑螟的肠道菌群门水平上,高温处理后的桑螟肠道中没有检测到酸杆菌门,但新增了梭杆菌门、浮霉菌门和软壁菌门;在属水平上,沃尔巴克菌属的丰度增加了26.38%,变化最大;在桑螟肠道优势菌群中,高温处理后变化最显著的为假单胞菌属,其次为普雷沃菌属,而节细菌属、噬纤维素菌属、埃希菌属、吉氏副拟杆菌属在高温处理后丰度下降,说明高温抑制了杆菌的繁殖,促进了其他菌的繁殖。本研究表明,高温处理后,桑螟肠道微生物的多样性和菌群结构发生了明显的变化,这种变化增加了桑螟的抗逆性,使其更好地适应高温环境。

To explore the relationship between stress-resistance physiology and gut microbiota of Glyphodes pyloalis Walker, high-throughput sequencing technique was performed to measure the sequence of 16 S r DNA-V4 variable region of gut bacteria in G. pyloalis before and after high temperature. The differences of bacterial communities in composition and bacterial diversity were analyzed, and the effects of gut microbiota changes on its stress-resistance physiology were clarified under high temperature. The results showed that at the level of phylum, Acidobacteria was not detected after the high temperature treatment, but Fusobacteria, Planctomycetes and Tenericutes were newly observed. At the level of genus, the abundance of Wolbachia increased by 26.38%, with the greatest change. Among the dominant bacterial communities in the gut of G. pyloalis, Pseudomonas had the most significant change after the high temperature treatment, then followed by Prevotella, while the abundances of Arthrobacter, Cellulophaga, Escherichia and Parabacteroides were decreased, indicating that high temperature inhibited the reproduction of bacillus but promoted the reproduction of other bacteria. This study shows that after the high temperature treatment, the diversity and structure of gut bacterial communities in G. pyloalis both have significant changes, which increase the stress-resistance of G.pyloalis to better adapt to high temperature environment.