DNA倍体检测对宫颈癌病灶中癌细胞增殖、侵袭活性的评估价值研究

The value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer

目的:研究DNA倍体检测对宫颈癌病灶中癌细胞增殖、侵袭活性的评估价值。方法:选择在本院接受手术切除的宫颈癌患者作为研究的宫颈癌组,选择同期接受子宫切除术的良性病变患者作为研究的对照组。手术前,取宫颈脱落细胞并进行DNA倍体检测;手术前,取宫颈组织并测定增殖基因、侵袭基因的表达量。结果:宫颈癌组患者脱落细胞中DNA倍体异常的细胞数目显著高于对照组;宫颈癌组患者宫颈癌组织中Id-1、Ki-67、TET1、S6K1、CatL、ILK的mRNA表达量显著高于对照组,HSG、MCPH1、p16、TIMP1、TIMP2的mRNA表达量显著低于对照组;DNA倍体异常细胞数目≥3个的宫颈癌组织中Id-1、Ki-67、TET1、S6K1、CatL、ILK的mRNA表达量显著高于DNA倍体异常细胞数目1~2个的宫颈癌组织,HSG、MCPH1、p16、TIMP1、TIMP2的mRNA表达量显著低于DNA倍体异常细胞数目1~2个的宫颈癌组织。结论:DNA倍体检测对宫颈癌病灶中癌细胞增殖、侵袭的活性具有评估价值。

Objective：To study the value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer.Methods：Patients with cervical cancer who underwent surgical resection in Yan＇an University Affiliated Hospital between March 2015 and May 2017 were selected as cervical cancer group of the research,and patients with benign lesions who underwent hysterectomy in Yan＇an University Affiliated Hospital during the same period were selected as the control group of the research.Before operation,cervical exfoliated cytology was taken for DNA ploid detection;before operation,the cervical tissue was taken to determine the expression of proliferation genes and invasion genes.Results：The number of exfoliated cells with abnormal DNA ploid in cervical cancer group was significantly higher than that in control group;Id-1,Ki-67,TET1,S6 K1,CatL and ILK mRNA expression in cervical cancer tissue of cervical cancer group were significantly higher than those of control group whereas HSG,MCPH1,p16,TIMP1 and TIMP2 mRNA expression were significantly lower than those of control group;Id-1,Ki-67,TET1,S6 K1,CatL and ILK mRNA expression in cervical cancer tissue with≥3 exfoliated cells with abnormal DNA ploid were significantly higher than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid whereas HSG,MCPH1,p16,TIMP1 and TIMP2 mRNA expression were significantly lower than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid.Conclusion：DNA ploid detection can be used to evaluate the proliferation and invasion of cancer cells in cervical cancer.