CT引导下经皮穿刺组织中TTF-1、NapsinA表达量与癌细胞恶性生物学的相关性

The correlation of TTF-1 and Napsin A expression in CT-guided percutaneous puncture tissue with the malignant biology of cancer cells

目的:研究CT引导下经皮穿刺组织中甲状腺转录因子-1(TTF-1)、天冬氨酸蛋白酶A(NapsinA)表达量与癌细胞恶性生物学的相关性。方法:选择2015年3月-2017年12月期间铜川市人民医院CT引导下经皮穿刺获得的肺组织,经病理诊断为肺癌组织78例、良性组织40例,抽提RNA后测定TTF-1、NapsinA、癌基因及上皮间质转化(EMT)基因的表达量。结果:肺癌组织中TTF-1、Pim1、Cmyc、Nr4a1、KLF4、N-cadherin的mRNA表达量显著高于良性组织、NapsinA、Rb、LKB1、HIPK2、Ecadherin的mRNA表达量显著低于良性组织;TTF-1高表达的肺癌组织中Pim1、C-myc的mRNA表达量显著高于TTF-1低表达的肺癌组织,Rb、LKB1的mRNA表达量显著低于TTF-1低表达的肺癌组织;NapsinA高表达的肺癌组织中HIPK2、E-cadherin的mRNA表达量显著高于NapsinA低表达的肺癌组织,Nr4a1、KLF4、N-cadherin的mRNA表达量显著低于NapsinA低表达的肺癌组织。结论:CT引导下经皮穿刺组织中高表达的TTF-1和低表达的NapsinA分别能够促进癌细胞的增殖及EMT。

Objective：To study the correlation of thyroid transcription factor-1（TTF-1）and Napsin A expression in CT-guided percutaneous puncture tissue with the malignant biology of cancer cells.Methods：The lung tissues that were obtained from CT-guided percutaneous puncture in Tongchuan People＇s Hospital between March 2015 and December 2017 were selected and pathologically diagnosed with lung cancer tissues（n=78）and benign tissues（n=40）,the RNA was extracted,and then the expression levels of TTF-1,NapsinA,oncogenes and epithelial-mesenchymal transition（EMT）genes were determined.Results：TTF-1,Pim1,C-myc,Nr4a1,KLF4 and N-cadherin mRNA expression levels in lung cancer tissues were significantly higher than those in benign tissues whereas NapsinA,Rb,LKB1,HIPK2 and E-cadherin mRNA expression levels were significantly lower than those in benign tissues;Pim1 and C-myc mRNA expression levels in lung cancer tissues with high TTF-1 expression were significantly higher than those in lung cancer tissues with low TTF-1 expression whereas Rb and LKB1 mRNA expression levels were significantly lower than those in lung cancer tissues with low TTF-1 expression;HIPK2 and Ecadherin mRNA expression levels in lung cancer tissues with high NapsinA expression were significantly higher than those in lung cancer tissues with low NapsinA expression whereas Nr4a1,KLF4 and N-cadherin mRNA expression levels were significantly lower than those in lung cancer tissues with low NapsinA expression.Conclusion：The high expression of TTF-1 and the low expression NapsinA in CT-guided percutaneous puncture tissue can promote the cancer cell proliferation and EMT respectively.