急性心肌梗死患者血清miR-208对血小板功能、炎症反应及心肌损伤程度的影响

Effect of serum miR-208 on platelet function,inflammatory response and myocardial injury in patients with acute myocardial infarction

目的:研究急性心肌梗死患者血清miR-208对血小板功能、炎症反应及心肌损伤程度的影响。方法:选择本院收治的急性心肌梗死患者及不稳定型心绞痛患者,分别作为研究的AMI组和UAP组;另取同期在安康市中心医院体检的健康志愿者作为对照组。测定miR-208的表达量、血小板参数、炎症反应细胞因子、心肌损伤标志分子。结果:AMI组和UAP组患者血清中miR-208的表达量以及PAF、TGF-β1、IL-10、TNF-α、sICAM1、sVCAM1、Myo、CK-MB、LDH、H-FABP的含量以及血小板表面C3aR、C5aR、GPⅡb/ⅡIa的表达强度均显著高于对照组且AMI组患者血清中miR-208的表达量以及PAF、TGF-β1、IL-10、TNF-α、sICAM1、sVCAM1、Myo、CK-MB、LDH、H-FABP的含量以及血小板表面C3aR、C5aR、GPⅡb/ⅡIa的表达强度均显著高于UAP组。结论:急性心肌梗死患者血清中miR-208的高表达能够加剧血小板激活、炎症反应活化、心肌损伤的程度。

Objective：To study the effect of serum miR-208 on platelet function,inflammatory response and myocardial injury in patients with acute myocardial infarction.Methods：Patients with acute myocardial infarction and those with unstable angina pectoris who were treated in Ankang Central Hospital between June 2015 and October 2017 were selected as the AMI group and UAP group of the research respectively;the healthy volunteers who received physical examination in Ankang Central Hospital during the same period were selected as the control group.The miR-208 expression,platelet parameters,inflammatory response cytokines and myocardial injury markers were measured.Results：Serum miR-208 expression,PAF,TGF-β1,IL-10,TNF-α,sICAM1,sVCAM1,Myo,CK-MB,LDH and H-FABP contents as well as platelet surface C3aR,C5aR and GPⅡb/ⅡIa expression intensity of AMI group and UAP group were significantly higher than those of control group（P〈0.05）,and serum miR-208 expression,PAF,TGF-β1,IL-10,TNF-α,sICAM1,sVCAM1,Myo,CK-MB,LDH and H-FABP contents as well as platelet surface C3aR,C5aR and GPⅡb/ⅡIa expression intensity of AMI group were significantly higher than those of UAP group（P〈0.05）.Conclusion：The high expression of serum miR-208 can aggravate the platelet activation,inflammatory response activation and myocardial injury in patients with acute myocardial infarction.