摘要
猪伪狂犬病是危害养猪业的重要病毒性传染病,如何鉴别疫苗接种动物和野毒感染动物是控制和净化该病的前提。本试验根据不同猪伪狂犬疫苗株gE基因缺失的特点,建立了一种猪伪狂犬病野毒病原核酸的PCR检测方法。该方法可以检出猪伪狂犬病野毒,而不能检出猪伪狂犬病疫苗株,实现了疫苗毒和野毒的鉴别检验,同时不能检测出猪常见病毒性传染病病原。最低检测限度为10~3个病毒核酸拷贝。在组织样品检测中不受宿主组织核酸干扰,能区分野毒感染动物组织和疫苗免疫动物组织,是一种值得推广的伪狂犬病毒野毒检测方法。
Porcine pseudorabigs is an important viral infectious disease that threatens the swine industry, and how to identify vaccinated animals and infected animals is a prerequisite for control and elimination of disease, A PCR method for detection wild porcine pseudorabies virus was established, based on the characteristics of gE gene deletion in different pseudorabies vaccine strains.The method only detected the wild porcine pseudorabies vires,but not thepseudorabies vaccine strain and other swine virus. The minimum detection limit of the method was 10^3 virus nucleic acid copies. In the tissue sample detection, it distinguished between infected animals and immune animal tissue, without interference by the tissue nucleic acid.
作者
马良
许磊
李晓冉
MA Liang;XU Lei;LI Xiao-ran(China Animal Husbandry Industry Co., LTD,Beijing 100070, china;Key Laboratory of Biological Products and chemical Drags for Animal, Ministry of Agricucture, Beijing 100095, China)
出处
《中国兽医杂志》
CAS
北大核心
2018年第2期41-43,共3页
Chinese Journal of Veterinary Medicine
关键词
猪伪狂犬病
野毒
鉴别
PCR
Porcine pseudorabies
wild virus
identification
PCR
