沙棘果对高脂膳食大鼠肝脏脂代谢的影响

Effect of seabuckthorn on liver lipid metabolism in high-fat diet rats

为了研究沙棘果对高脂膳食大鼠肝脏脂代谢的影响,以60只雄性Wistar大鼠为研究对象,随机分为5组,对照组、高脂模型组、沙棘果低、中和高剂量组。对照组给予基础饲料,其余各组给予高脂饲料喂养,同时试验组每日用不同浓度的沙棘果匀浆液灌胃大鼠。4周后处死动物,采集肝脏,分别测定肝脏脂质、脂代谢相关酶及抗氧化指标,光镜下观察肝脏组织病理改变。结果:沙棘果匀浆液可显著降低肝脏总胆固醇(TC)水平,增加高密度脂蛋白(HDL-C)水平;使肝脏组织总脂酶(LPS)、肝脂酶(HL)和脂蛋白脂酶(LPL)活性增强,MDA生成量减少。结论:沙棘果可降低高脂膳食大鼠肝脏脂质水平,提高肝脏脂代谢酶活性,增强抗氧化能力,减缓肝细胞的脂质过氧化。

To study the effects of seabuckthorn on liver lipid metabolism in high-fat diet rats. Sixty Wistar rats were divided into five groups, the control group, the high fat diet group and seabuckthorn low-, medium- and high-dose groups. The control group was fed with basic diet, and other groups were fed with high fat diet during the experiment. Then rats in both control group and high fat/ diet group were given distilled water and those in low-, medium- and high- dose treated group were treated with different doses of sea- buckthorn. And then TC. TG, HDL-C, LPS, HL, LPL, SOD and MDA levels were measured. The pathological changes of liver were observed. Compared with the high fat diet group, seabuckthorn reduced TC, increased HDL-C, regulated the activities of LPS, HL and LPL, and reduced the content of MDA in high-fat diet rats. Seabuckthorn could reduce hepatic lipids, improve the activities of lipid metabolism enzymes in liver, increase the antioxidant capacity and inhibit lipid peroxidation of liver.