增生型糖尿病视网膜病变患者血清及玻璃体中IL-19和VEGF表达水平的研究

The expression of IL-19 and VEGF in serum and vitreous of patients with proliferative diabetic retinopathy

目的探讨增生型糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)患者血清及玻璃体中白细胞介素-19(interleukin-19,IL-19)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达及临床意义。方法选取37例PDR患者和40例孔源性视网膜脱离(rhegmatogenous retinal detachment,RRD)患者分别作为实验组和对照组,采用酶联免疫吸附试验(ELISA)检测2组患者血清和玻璃体液中IL-19、VEGF的含量,PDR组患者检测空腹血糖(fasting blood glucose,FBG)、糖化血红蛋白(glycosylatedhemoglobin,Hb Alc)水平。结果 PDR组患者血清和玻璃体液中的IL-19和VEGF水平均显著高于对照组,差异均有统计学意义(t=8.747、16.437;t=10.417、14.978,均P<0.01),且两者之间呈正相关关系(r=0.394,P<0.05;r=0.574,P<0.01)。PDR组患者玻璃体液中IL-19和VEGF水平均高于血清水平,差异均有统计学意义(均P<0.05)。PDR组玻璃体中VEGF表达水平与FBG、Hb Alc均呈正相关性(r=0.361、0.672,均P<0.05);PDR组患者IL-19表达水平与糖尿病病程、FBG、Hb Alc均无明显相关性(均P>0.05)。结论 IL-19和VEGF在PDR的发病机制中扮演着重要角色;IL-19与VEGF正相关,可能参与了PDR新生血管病变的发生发展,有望为PDR的治疗带来更多的靶点。

To investigate the expression and clinical significance of interleukin-19（IL-19） and vascular endothelial growth factor（VEGF） in the serum and vitreous of patients with proliferative diabetic retinopathy（PDR）,the levels of IL-19 and VEGF in the serum and vitreous fluid from 37 PDR patients（test group） and 40 patients with rhegmatogenous retinal detachment（RRD）（control group） were measured by ELISA. The patients in PDR group were tested for fasting blood glucose（FBG） and glycosylated hemoglobin（Hb Alc） levels. Data showed that the levels of IL-19 and VEGF in the serum and vitreous fluid of PDR group were all significantly higher than those of control group（t=8.747, 16.437; t=10.417, 14.978, all P0.01）, and there was a positive correlation between them（r=0.394,P0.05; r=0.574, P0.01）; the levels of IL-19 and VEGF in vitreous fluid were all higher than that in serum of PDR group（all P0.05）. The level of VEGF in vitreous was positively correlated with FBG and Hb Alc in PDR group（r=0.361, r=0.672, all P0.05）; the level of IL-19 was not correlated with the duration of diabetes, FBG level or Hb Alc level（all P0.05） in PDR group. In conclusion, IL-19 and VEGF are positive correlated and play criticalroles in the pathogenesis of PDR; IL-19 may beinvolved in the occurrence and development of PDR neovascularization, thus may provide more therapeutic targets for PDR.