高迁移率族蛋白1基因多态性与代谢综合征的相关性

The association of high-mobility group box-1 protein genetic polymorphism with metabolic syndrome

目的探讨福建地区高迁移率族蛋白1(HMGB1)基因多态性与代谢综合征(MS)的关系。方法共纳入2015年2月-2016年2月期间连续住院治疗的受试者1005例,其中MS组450例,非MS组555例。采用病例对照关联分析研究方法,探讨HMGB1基因单核苷酸多态性rs2249825、rs1045411、rs1412125与MS及其各组分的关系。应用直接计算法计算等位基因及基因型频率,利用HaploView4.2软件分析其单倍体,非条件Logistic回归分析HMGB1各位点基因型与MS的关系。结果 (1)HMGB1基因位点rs2249825、rs1045411、rs1412125在共显性、显性、隐性3种遗传模式下与MS相关性无统计学意义(均P>0.05)。HMGB1基因rs2249825-rs1045411单倍体CA在MS组与非MS组分布差异有统计学意义(P=0.027)。(2)HMGB1位点rs2249825的共显性模式基因型GC及显性模式基因型GG+GC是高三酰甘油发生的影响因素(分别OR=1.573,1.558,分别P=0.041、0.038)。HMGB1基因rs2249825-rs1045411单倍体GA、CA在高三酰甘油组与非高三酰甘油组分布差异有统计学意义(分别P=0.007,0.030)。(3)HMGB1位点rs2249825的显性模式基因型GG+GC(OR=0.711)、rs1045411的共显性模式基因型AA(OR=0.457)及隐性模式基因型AA(OR=0.438)和rs1412125的共显性模式基因型CC(OR=0.529)及显性模式基因型CC+CT(OR=0.714)是高血压发生的影响因素(均P<0.05)。结论 HMGB1基因多态性与MS危险因素成分中的高三酰甘油、高血压具有一定相关性。

Objective To study the relationship between high-mobility group box-1 protein （HMGB1） genetic polymorphism and metabolic syndrome {MS） in Fujian province. Methods A total of 1005 participants were included in the research between February 2015 and February 2016. These participants were divided into MS group （n = 450） and control group （non-MS group, n=555）. The case-control study method was used to evaluate associations of HMGB1 gene single nucleotide polymorphism （rs2249825, rs1045411 and rs1412125 ）with MS and it＇s components. The direct calculation method was used to calculate the alleles and genotype frequency. HaploView4.2 software was used to analyze the haploid of HMGB1 gene, and unconditioned Logistic regression analysis was used to analyze the relationships between genotypes of HMGB1 and MS. Results （1）The co-dominant, dominant and recessive genetic models of HMGB1 genetic locus rs2249825, rs1045411 and rs1412125 showed no significant correlation with MS （all P〉0.05）. There was a significant difference in the distribution of haploid CA of HMGB1 gene rs2249825-rs1045411 between the MS group and non-MS group （P=0. 027）. （2）The co-dominant genotype GC and dominant genotype GG＋GC of HMGB1 genetic locus rs2249825 were significant influencing factors for high triglyceride （OR= 1. 573, 1. 558, P= 0. 041, 0. 038, respectively）. There were significant differences in the distributions of haploid GA and CA of HMGB1 gene rs2249825-rs1045411 between the high triglyceride and non-high triglyceride group （P=0. 007, 0. 030; respectively）. （3）The dominant genotype GG＋GC of HMGB1 genetic locus rs2249825（OR=0. 711）, co-dominant genotype AA （OR=0. 457） and recessive genotype AA （OR=0. 438） of rs1045411, codominant genotype CC （OR=0. 529） and dominant genotype CC＋CT （OR=0. 714） of rs1412125 were all significant influencing factors for hypertension （all P〈0.05）. Conclusion HMGB1 genetic polymorphism was related to high triglyceride and hypertension.