紫外-微波复合诱变选育溶煤菌株及其溶煤产物分析

UV/Microwave Compound Mutation Breeding Coal Solubilization Strains and Analysis of its Product Composition

生物转化是煤炭清洁温和利用的有效途径之一.为提高神府煤的生物溶解率及产物定向转化的选择性,分别用沙门氏菌、黄杆菌、大肠杆菌、枯草杆菌、金黄色葡萄球菌、黑曲霉菌及云芝菌对神府煤进行溶解.以最佳溶煤菌为出发菌株,利用紫外-微波复合诱变,选育更高效的溶煤菌株进行煤的生物转化,用FTIR和GC/MS对溶煤产物进行了分析.结果表明,紫外-微波复合诱变对云芝菌具有明显的诱变效应,复合诱变后的云芝菌对神府煤具有最好的溶解效果,其溶煤率从原来的41.3%提高到54.2%.FTIR和GC/MS分析表明,神府煤经生物转化,产物的芳香类物质相对含量降低,而烷烃、酰胺、烯烃、醇及醚类物质含量增多.

Bioconversion is one of the effective,clean and moderate ways to utilize coal. To increase Shenfu coal bio-solubilization and selectivity of its bioconversion products,Shenfu coal was dissolved respectively by coal solubilization strains such as Salmonella,F. odoratum,E. coli,Bacillus subtilis,Staphylococcus aureus,Aspergillus and Coriolus versicolor. The most optial coal solution strain was used as starting strain,and was breeded by UV/microwave compound mutation,the biodegradation products were characterized by FTIR and GC/MS analysis. The results showed that,Coriolus versicolor have the highest ability for coal solubilization,and the coal solubilization rate of Coriolus versicolor increased from 41. 3% to 54. 2% after UV/microwave compound mutation. FTIR and GC/MS analysis further show the biodegradation products of aromatic substances relatively decrease,and paraffin,amide,olefins,alcohols,and ethers matter content increase.