口腔、口咽鳞状细胞癌中HPV感染与EGFR之间的相关性研究

The Correlation between HPV Infection and EGFR in Oral and Oropharyngeal Squamous Cell Carcinoma

目的:通过检测人乳头状病毒(human papilloma virus,HPV)感染相关蛋白P16与表皮生长因子受体(epidermal growth factor receptor,EGFR)在口腔、口咽鳞癌中的表达,探索HPV感染与EGFR之间的关系,以及探讨P16、EGFR蛋白在口腔、口咽鳞癌中的表达差异及其临床病理意义。方法:收集53例口腔鳞癌和35例口咽鳞癌组织标本及其临床资料,采用免疫组化法检测P16、EGFR蛋白的表达情况,并结合临床资料分析临床意义。同时采用t检验、χ~2检验和分类变量的关联性分析法分析数据。结果:本组88例患者中P16阳性率为40.9%,EGFR阳性率为69.3%,P16与EGFR在口腔、口咽鳞癌中具有相关性(P<0.05,χ~2=14.77,r=0.379)。口腔鳞癌患者P16阳性率为22.6%(12/53),口咽鳞癌患者阳性率为68.6%(24/35),两者差异有统计学意义(P<0.000),口腔鳞癌患者EGFR阳性率为56.6%(30/53),口咽鳞癌患者阳性率为88.6%(31/35),两者差异有统计学意义(P<0.001)。P16阳性组患者的吸烟情况、肿瘤分化程度、复发情况和患者性别与阴性组患者比较,差异均有统计学意义(均P<0.05)。EGFR阳性组和阴性组仅在患者性别情况比较上,有统计学差异(P<0.05)。结论:在口腔、口咽鳞癌中P16的表达与EGFR呈正相关,说明HPV感染状态可能影响EGFR的表达,P16、EGFR在口腔与口咽上的表达分布存在差异。P16表达与肿瘤复发、分化程度、患者性别及吸烟情况有关。

Objective： To investigate the relationship between HPV infection and EGFR by detecting the correlation between HPV infection-related protein P16 and EGFR,and to explore the difference of P16 and EGFR protein expression and the clinicopathological features in oral and oropharyngeal squamous cell carcinoma. Methods： the tissue sample of 53 oral squamous cell carcinoma,and 35 oropharyngeal squamous cell carcinoma were collected. Immunohistochemistry was used to detect the expression of P16 and EGFR protein in oral and oropharyngeal squamous cell carcinoma. The data were analyzed using the t test,the χ~2 test and the relevance analysis of categorical variables. Results： The positive rate of P16 was 40. 9% and the positive rate of EGFR was69. 3%. P16 and EGFR were correlated in oral and oropharyngeal squamous cell carcinoma（ P 0. 05,χ~2= 14. 77,r = 0.379）. The positive rate of P16 was 22. 6%（ 12/53） in oral squamous cell carcinoma and 68. 6%（ 24/35） in patients with oropharyngeal squamous cell carcinoma,the difference was statistically significant（ P 0. 001）,The positive rate of EGFR was 56. 6%（ 30/53） in oral squamous cell carcinoma,but was 88. 6%（ 31/35） in oropharyngeal squamous cell carcinoma,the difference was statistically significant（ P 0. 001）. P16 positive group of patients with smoking,tumor differentiation,recurrence and gender compares with negative group,the difference was statistically significant（ P 0. 05）. Comparison of EGFR positive group and negative group on gender exist the difference that was statistically significant（ P 0. 05）.Conclusion： In oral and oropharyngeal squamous cell carcinoma,there exists a positive link between P16 and EGFR. And HPV infection maybe associate with EGFR. The expression of P16 and EGFR were different in oral and oropharyngeal squamous cell carcinoma. P16 expression was associated with tumor recurrence,differentiation,patient sex and smoking.