巴西橡胶树HbMYBs转录因子的克隆及表达分析

Cloning and Expression Profiling of Transcription Factors HbMYBs of Hevea brasiliensis

通过同源序列比对,笔者克隆了巴西橡胶树5个与抗逆相关的HbMYBs基因,根据其与拟南芥同源基因的相似性,分别命名为HbMYB15,HbMYB21,HbMYB24,HbMYB35和HbMYB75,其阅读框长度分别为651,984,999,1 095,933 bp。生物信息学分析结果表明,这5个HbMYBs基因编码的蛋白序列都包含MYB家族结构域,属于R2R3类MYB转录因子。酵母自激活实验结果显示这5个HbMYBs蛋白均具有转录活性,具有转录因子特征。荧光定量PCR分析结果表明,创伤、乙烯处理和茉莉酸处理对这5个HbMYBs基因中有不同程度的诱导作用,这些基因有可能参与乙烯和茉莉酸信号相关的植物防御应答。

Hevea brasiliensis is the main source of natural rubber.MYB gene family plays an important role in plant growth,development and responses under environmental stresses.Through homologous sequence alignment,five HbMYBs related genes were cloned in H.brasiliensis and termed as HbMYB15,HbMYB21,HbMYB24,HbMYB35 and HbMYB75 according to the similarity with the homologous gene in Arabidopsis.The ORF（open reading frame） lengths of these five HbMYBs are 651 bp,984 bp,999 bp,1 095 bp and 933 bp,respectively.Bioinformatics analysis showed that all the HbMYBs protein contain MYB conserved domains and belong to R2R3 type MYB transcription factor.Yeast self-activation experiments showed that the five HbMYBs proteins had transcriptional activity,indicating that the five HbMYBs genes are potential transcription factors.The results of quantitative RT-PCR showed that the five HbMYBs genes were regulated by treatment of wounding,ethylene or jasmonic acid,indicating that these genes may be involved in the plant defense response related to ethylene and jasmonic acid signaling pathway.