高效液相色谱法测定氰尿酸的纯度

Determination of the purity of cyanuric acid by HPLC

采用高效液相色谱法测定氰尿酸的纯度,测定条件:色谱柱为C18(250 mm×4.6mm,5μm),pH=4.5磷酸盐缓冲液-乙腈(85∶15)为流动相,流速为1.2 mL/min,测定波长为213 nm。结果表明,在0.15~1.35 mg/mL浓度范围内,峰面积与浓度呈良好的线性关系,最低检测限为0.50μg/mL。该方法简便、快速、准确,适于氰尿酸纯度的测定。

The purity of cyanuric acid was determined by HPLC. The determination conditions were that the chromatographic column was C_（18）（ 250 mm×4.6 mm, 5 μm） with the mobile phase of pH = 4.5 phosphate buffer-acetonitrile（ 85∶15）,the flow was 1.2 mL/min and detection wavelength was 213 nm. The results show that in the concentration range of 0.15 -1.35 mg/mL, the peak area is in a good linear relationship with the concentration, and the minimum detection limit is 0.50μg/mL. The method is simple, rapid and accurate, and suitable for the determination of the purity of cyanuric acid.