阿霉素偶联氧化锌量子点的制备及其对肺癌A549细胞增殖抑制作用的研究

The Study of Doxorubicin Coupled ZnO QDs on the Inhibition of Lung Cancer A549 Cells Proliferation

目的开发一种具有减毒增效作用的载阿霉素的ZnO量子点(ZnO QDs)。方法制备TK键偶联阿霉素(DOX)/ZnO QDs的药物载体,并通过DLS考察粒径的变化、FT-IR鉴定化学结构、ζ电势考察每一步反应后量子点表面电势的变化等多种方法加以表征,以确认载体的形成,最后采用噻唑蓝比色法(MTT法)考察其对人肺癌A549细胞增殖的抑制作用。结果成功构建偶联阿霉素的ZnO QDs,体外释放实验证明载体具有酸敏释药的性质,MTT结果显示其对A549细胞具有较强的杀伤效应。结论阿霉素偶联ZnO QDs可以在肿瘤细胞内有效释药,并产生较强的细胞杀伤作用,具有进一步开发成体内抗肿瘤递药系统的潜力。

Objective To develop ZnO quantum dots （QDs） loading doxorubicin which has lower toxicity and higher effect on anti-tumor treatment. Methods The drug-loaded QDs were prepared by coupling ZnO QDs with Doxorubicin through TK linker, and were confirmed by DLS, FT-IR and zeta potential. Finally the anti-proliferation activities of doxorubicin-ZnO QDs against lung cancer A549 cells were evalu-ated by MTT assay. Results The doxorubicin-ZnO QDs were prepared and showed good efficiency of killing A549 tumor cells. Conclusions The doxorubicin-ZnO quantum dots could release the drug in A549 tumor cell and have the potency of developing into a new anticancer drug delivery system in vivo.