两种制备鸡肝癌细胞染色体方法的比较

Comparison of two methods for chromosome preparation from LMH cells

目的建立两种鸡肝癌细胞系(LMH)染色体的制备方法,并对两种方法进行比较。方法将24瓶鸡肝癌细胞分成两组,一组在传代前用明胶铺满培养瓶底,另一组直接接种细胞于培养瓶中,72 h后明胶处理过的一组采用原位法制备核型,另一组采用滴片法制备核型。结果原位法和滴片法制备的鸡肝癌细胞染色体数目、染色体相对长度及染色体形态无明显差异,但原位法制备的核型较滴片法多,步骤较滴片法简单、稳定。结论原位法制备鸡肝癌细胞染色体优于滴片法。

Objective To establish and compare two methods for chromosome preparation from LMH cells. Methods Twenty-four bottles of LMH cells were divided into two groups, one group was treated by 0.1% gelatin before cells subcultured, the other group was cultured directly in the bottle. After 72 hours, in-situ method was used for preparation of karyotypes of cells in gelatin group, and the other group was prepared with drop method. Results There was no significant difference in chromosome numbers and relative length between in-situ method and drop method. While, more karyotypes were found with in-situ method and it costed less steps and seemed more stable when compared with drop method. Conclusion In situ method is superior to drop method for chromosome preparation from LMH cells.