人γ干扰素诱导蛋白16与子痫前期发病的关系

The relationship between human-induced protein 16 and preeclampsia

目的探讨人γ干扰素诱导蛋白16(IFI16)与子痫前期(PE)的关系。方法选择2015年6月~2017年3月广州市番禺区何贤纪念医院(以下简称"我院")收治的PE患者125例作为研究组,另外选择同期于我院进行体检的健康孕妇53例作为对照组。分别采用酶联免疫吸附测定法和蛋白免疫印迹法检测孕妇血清中IFI16水平和胎盘中IFI16蛋白的表达,并用Pearson相关分析PE患者血清中IFI16水平及胎盘中IFI16蛋白表达的相关性。结果研究组孕妇血清中IFI16水平及胎盘中IFI16蛋白的表达水平均明显高于对照组(t=17.580、16.135,均P<0.01);与轻度PE患者相比,重度患者血清中IFI16水平及胎盘中IFI16蛋白的表达水平均明显升高(t=18.883、14.845,均P<0.01);早发型和晚发型PE患者血清中IFI16水平及胎盘中IFI16蛋白的表达水平之间的差异无统计学意义(t=0.360、0.840,均P>0.05);PE患者血清中IFI16水平及胎盘中IFI16蛋白的表达水平呈正相关(r=0.725,P<0.05)。结论 PE患者血清中IFI16和胎盘中IFI16蛋白表达水平异常升高,并且与疾病发展密切相关,可以作为临床诊断指标,并且为治疗PE提供新的靶点。

Objective To explore the relationship between human interferon-inducible protein 16（IFI16） and the onset of preeclampsia（PE）. Methods From June 2015 to March 2017, 125 patients with PE in Panyu District He Xian Memorial Hospital of Guangzhou（ ＂our hospital＂ for short） were selected as the research group. In addition, 53 cases of healthy pregnant women in our hospital during the same period were treated as control group. The levels of IFI16 in serum and IFI16 protein expression in the placenta of pregnant women were detected by enzyme-linkedimmunosorbent assay and Western blotting respectively. The correlation between the level of IFI16 in serum and the expression of IFI16 in the placenta of pregnant women with PE was analyzed by Pearson. Results The levels of IFI16 in serum and the expression of IFI16 in the placenta of research group were significantly higher than those in the control group（t=17.580, 16.135, all P 〈 0.01）. Compared with patients of mild PE, the level of IFI16 in serum and the expression of IFI16 in the placenta of patients with severe PE significantly increased（t=18.883, 14.845, all P 〈 0.01）. There were no statistically significant differences of the level of IFI16 in serum and the expression of IFI16 in the placenta between early onset and late onset（t=0.360, 0.840, all P 〉 0.05）. The expression levels of IFI16 in the serum of PE patients were positively correlated with the expression level of IFI16 in the placenta（r=0.725, P 〈 0.05）. Conclusion The level of IFI16 in serum and the expression of IFI16 in the placenta of patients with PE are abnormally elevated, they are closely related to the disease development, which can be used as a clinical diagnosis of indicator and provide a new target for the treatment of PE.