基于氮掺杂多孔碳及PoPD电化学免疫传感器的构建

An electrochemical immunosensor for ultrasensitive detecton of alpha-fetoprotein based on nitrogen-doped porous carbon and PoPD

氮掺杂多孔碳具有良好的生物相容性,聚邻苯二胺具有丰富的氨基以及亚氨基,能提高电化学免疫传感器的性能。本文构建了一种具有较高选择性和稳定性以及灵敏性的无标记型电化学免疫传感器用于检测甲胎蛋白(AFP)。基于氮参杂多孔碳/聚邻苯二胺修饰电极表面,采用循环伏安(CV)及交流阻抗法(EIS)研究了修饰电极表面的电化学特性。在优化的条件下,该电化学免疫传感器的相应电流与AFP的浓度在0.005-100 ng/mL范围内呈现良好的线性关系,检测限为0.0006 ng/mL。新型电化学免疫传感器可为临床应用提供潜在的应用。

In this study, we constructeda label-free electrochemical immunosensor for highly selective detection袁 stability andsensitive detection of alpha-fetoprotein （AFP） based on PoPD and N-doped porous carbon. The electrochemical characteristicswith the modified electrode surface were studied by cyclic voltammetry （CV） and electrochemical impedance spectroscopy （EIS）measurements. The using of N-doped porous carbon greatly enhanced the biology catalytic activity due to its large specificsurface and excellent biocompatibility. PoPD has large amino which can improved its ability to fixed anti-AFP. Under theoptimized conditions, the results show that the immunosensor have excellent electrochemical properties witha wide concentrationrange from 0.005 to 100 ng/mL anda detection limit of 0.0006 ng/mL. The new type of electrochemical immunosensor mayprovide potential applications for the clinic application.