摘要
Phanerochaete chrysosporium was selected as the production strain of laccase,and the effects of stirring speed,ventilation volume,culture temperature,inoculation amount and initial p H of medium on laccase production by liquid fermentation in cylinder were studied. On the basis of single factor test,an orthogonal test was carried out to find optimal conditions for laccase production P. chrysosporium through liquid fermentation. These results showed that the stirring speed of fermentation cylinder had the highest effect on laccase production,and the optimal conditions were shown as follows： the temperature at 28 ℃,the rotating speed at 300 r/min,the ventilation volume of 5 L/min（ ventilation ratio of 1.0 vvm）,the initial p H of medium of 5,and the inoculation amount of 15%,which gave the highest laccase level of 14. 86 U/ml.
Phanerochaete chrysosporium was selected as the production strain of laccase,and the effects of stirring speed,ventilation volume,culture temperature,inoculation amount and initial p H of medium on laccase production by liquid fermentation in cylinder were studied. On the basis of single factor test,an orthogonal test was carried out to find optimal conditions for laccase production P. chrysosporium through liquid fermentation. These results showed that the stirring speed of fermentation cylinder had the highest effect on laccase production,and the optimal conditions were shown as follows： the temperature at 28 ℃,the rotating speed at 300 r/min,the ventilation volume of 5 L/min（ ventilation ratio of 1.0 vvm）,the initial p H of medium of 5,and the inoculation amount of 15%,which gave the highest laccase level of 14. 86 U/ml.
作者
haixia liu
li zhang
liang qiao
Haixia LIU;Li ZHANG;Liang QIAO(College of Animal Science and Technology,Jiangsu Agri-Animal Husbandry Vocational College,Taizhou 225300,chin)
基金
Supported by Project of Jiangsu Agri-Animal Husbandry Vocational College(NSFPT201601)
