46例肥胖者肠道肠杆菌菌群与内毒素的调查研究

Investigation of intestinal flora and endotoxin in 46 obese patients

目的:探讨肥胖的肠道肠杆菌菌群与血浆内毒素之间的关系。方法:根据BMI和内毒素(LPS)筛选出肥胖者,并对其粪便进行培养,对内毒素与细菌菌落计数进行线性回归分析。对培养菌落做16Sr DNA片段的PCR并电泳。结果:46例肥胖者粪便培养出大肠埃希菌42例,非大肠埃希菌4例;同时培养出2种肠杆菌6例。42例肥胖者粪便同时培养出大肠埃希菌和肠球菌的2例;培养出大肠埃希菌和酵母菌的2例。LPS所测的值与肠道菌落计数大小趋势呈现正相关。大肠埃希菌用VITEK2和16Sr DNA PCR电泳结果一致。结论:肥胖者肠道菌群以肠杆菌中大肠埃希菌为主,辅以阴沟肠杆菌、肺炎克雷伯菌、肠球菌和酵母菌,其菌量多少与LPS大小趋势一致。

Objective：To evaluate the associations between obesity intestinal flora with the plasma toxin. Methods： According to BMI and endotoxin（LPS）, obese patients were selected and their faeces were cultured, and the endotoxin and bacterial colony count were analyzed in linear regression.PCR and electrophoresis of 16 Sr DNA fragments were conducted for the cultivation of colonies. Results： In 46 cases of obesity, 42 cases Escherichia coli（E. coli） was isolated from stool, and non Escherichia coli was found in 4 cases.A total of 6 cases of the two types of enterobacterium were cultured in 46 obese individuals. Two cases of E. coli and Enterococcus were developed in 42 cases of obese human excrement. In 42 cases of obese human feces, two cases were developed for E. coli and yeast. The value of LPS was positive correlation with the intestinal colony count. The results of VITEK2 and 16 Sr DNA PCR in Escherichia coli were consistent. Conclusion： Obesity intestinal enterobacteriaceae flora were Escherichia coli, supplemented by Enterobacter cloacae, Klebsiella pneumoniae, Enterococcus bacteria and yeast, the amount was consistent with size of LPS.