支气管哮喘患儿血清中Eotaxin、IL-13的表达水平及临床意义

Expression and clinical significance of Eotaxin,IL-13 in the serum of children with bronchial asthma

目的:探讨嗜酸性粒细胞趋化因子(Eotaxin)和白细胞介素13(IL-13)在支气管哮喘患儿发病过程中的作用及相互关系。方法:选择24例支气管哮喘患儿(哮喘组)和30名健康儿童(健康对照组)作为研究对象。用酶联免疫吸附实验(ELISA)法检测各组儿童血清中Eotaxin、IL-13水平,哮喘患儿组同时行肺功能测定。结果:哮喘患儿组急性发作期、哮喘缓解期血清中Eotaxin、IL-13表达水平与健康对照组比较,差异均有统计学意义(P<0.05)。在哮喘患儿组,急性发作期、哮喘缓解期血清中Eotaxin、IL-13彼此均成正相关。在哮喘急性发作期、哮喘缓解期血清中Eotaxin、IL-13与一秒钟用力呼气量(FEV1)、呼气高峰流量(PEF)均呈负相关。结论:Eotaxin、IL-13在支气管哮喘发病过程中彼此相互影响,共同参与哮喘患儿病理生理变化过程;Eotaxin、IL-13可作为哮喘患儿除临床症状、体征外评定病情严重程度、评定疗效的客观指标。

Objective： To explore the interaction and relationship of Eotaxin and IL-13 in children with bronchial asthma. Methods： 24 children with bronchial asthma（ study group） and 30 healthy children（ healthy control group） were selected. IL-13 and Eotaxin levels of the two groups were measured by sandwich ELISA,and the pulmonary function of patients in study group was detected at the same time. Results： There was a significant difference in serum Eotaxin and IL-13 expression of acute attack stage and asthma remission stage compared with healthy control group（ P〈0. 05）. In the children with asthma,Eotaxin and IL-13 were positively related to each other in the acute phase and the asthma remission stage. The serum levels of Eotaxin and IL-13 were negatively correlated with the one second forced expiratory volume（ FEV1） and peak expiratory flow（ PEF） in the acute and remission stage of asthma. Conclusion：Eotaxin and IL-13 interact with each other during the pathogenesis of bronchial asthma and participate in the pathophysiological changes of children with asthma. Eotaxin and IL-13 can be used as an objective index to assess the severity of the disease and evaluate the effect of asthma in children with asthma.